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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IH(P) | M | Culture Supernatant | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB3070 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Granzyme B Antibody, clone GrB-7 |
| Product Information | |
|---|---|
| Format | Culture Supernatant |
| Presentation | Liquid |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Granzyme B using this Anti-Granzyme B Antibody, clone GrB-7 validated for use in WB, IH(P). |
| Key Applications |
|
| Application Notes | Immunoblotting Immunohistochemistry of granzyme B-expressing lymphocytes in sublimate sections and formalin-fixed paraffin-embedded tissues (1:20 dilution). Does not react with other cell types. Cannot be used on frozen sections. Note on the use of MAB3070 on paraffin sections: Tissue slides should be pretreated with an antigen retrieval method, such as treatment with 0.1M sodium citrate for 10 min at 100°C. A working dilution of 1:20 is advised, but optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Clone | GrB-7 |
| Host | Mouse |
| Specificity | Monoclonal antibody recognizes the 33 kDa granzyme B. Reacts specifically with human serine protease granzyme B. Does not cross-react with human granzyme A. Granzyme B localizes in cytoplasmic granules and can be used as a marker for NK cells and activated cytotoxic T-cells. |
| Isotype | IgG2a |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface nonself antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein encoded by this gene is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response. |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P10144 # This enzyme is necessary for target cell lysis in cell- mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. SIZE: 247 amino acids; 27688 Da SUBCELLULAR LOCATION: Cytoplasmic granule. Note=Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells. SIMILARITY: SwissProt: P10144 ## Belongs to the peptidase S1 family. Granzyme subfamily. & Contains 1 peptidase S1 domain. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C for up to 12 months. |
| Packaging Information | |
|---|---|
| Material Size | 250 µL |