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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| A | ELISA, ICC, IF, IHC, WB | M | Ascites | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB3120 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Cre Recombinase Antibody, clone 2D8 |
| Background Information | Cre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the "phage" integrase family. This antibody can be used to determine the success of gene manipulations in transgenic animals. |
| Product Information | |
|---|---|
| Format | Ascites |
| Control |
|
| Presentation | Ascites fluid. Contains no preservative |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Cre Recombinase Antibody, clone 2D8 is a well characterized monoclonal antibody. Proven to reliably detect Cre Recombinase, this mAb is validated for use in ELISA, IC, IF, IH & WB and is backed by multiple publications. |
| Key Applications |
|
| Application Notes | Western Blot (natural and recombinant protein): A 1:500-1:5,000 dilution was used in a previous lot. Natural Cre recombinase from Bacteriophage P1 migrates at 38.5 kDa by SDS-PAGE. Immunofluorescence: A previous lot of this antibody was used on IF. Immunohistochemistry: A 1:500-1:5,000 dilution of a previous lot was used in IH. ELISA: A 1:500-1:5,000 dilution of a previous lot was used in ELISA. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Cre recombinase (aa 77 to 343) fusion protein |
| Clone | 2D8 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Cre recombinase. The epitope is located between aa 77 and 343. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Purification Method | Unpurified |
| UniProt Number |
|
| Molecular Weight | 38 kDa |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20ºC from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |