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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC, IP, WB, IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB3121 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Bcl-XL and BclXs Antibody, clone 7B2.5 |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified immunoglobulin - Ig fraction. Liquid in borate buffer saline, pH 8.2. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Bcl-XL & BclXs using this Anti-Bcl-XL & BclXs Antibody, clone 7B2.5 validated for use in FC, IP, WB, IH. |
| Key Applications |
|
| Application Notes | Flow cytometry: Immunoprecipitation (Cheng, 1996; Gottschalk, 1996) Immunohistochemistry (Foreman, 1996; Wrone-Smith, 1995) Western blotting: ( 0.5 μg/mL) Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant Bcl-XS. |
| Clone | 7B2.5 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Apoptosis, or programmed cell death, is a well-documented phenomenon in many cellular systems (Cohen, 1991). It plays a key role in tissue and organ development, as well as in adult tissues during cell turnover. Apoptosis can be induced by a variety of internal and external stimuli including growth factor deprivation, cytokine treatment, antigen-receptor engagement, cell-cell interactions, irradiation and glucocorticoid treatment (Cohen & Duke, 1992). Bcl-2 and one of its homologues, Bcl-XL, protect cells from apoptosis (Nunez & Clarke, 1994; Cory, 1995), while other homologues of Bcl-2 such as Bax, Bad and Bak have been shown to enhance apoptosis (Oltvai, 1993; Farrow, 1995; Chittenden, 1995; Kiefer, 1995). Bcl-XL has been shown to block apoptosis which is induced by a variety of stimuli and, under certain conditions, offers greater protection against apoptosis than Bcl-2 (Boise, 1993; Gottschalk, 1994; Gonzalez-Garcia, 1995; Dole, 1995; Shimizu, 1995). In contrast, Bad and Bax inhibit the protective functions of Bcl-XL and Bcl-2, respectively. Although heterodimerization between Bcl-XL, Bad and Bcl-2/Bax was originally thought to be essential for the differential anti-apoptotic activity of Bcl-XL and Bcl-2 (Oltvai, 1993; Yin, 1994); other results suggest that the formation of heterodimers may not be necessary for this death-repressing activity Cheng, 1996; Gottschalk, 1996). This antibody recognizes Human Bcl-XL (Mr 29 kDa) and Bcl-Xs (Mr 21 kDa). |
| Isotype | IgG3 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q07817 # Potent inhibitor of cell death. Isoform Bcl-X(L) anti- apoptotic activity is inhibited by association with SIVA isoform 1. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, cytochrome c, from the mitochondrial membrane. The Bcl-X(S) isoform promotes apoptosis. SIZE: 233 amino acids; 26049 Da SUBUNIT: Bcl-X(L) forms homodimers, and heterodimers with BAX, BAK and BCL2. Heterodimerization with BAX does not seem to be required for anti-apoptotic activity. Also interacts with BAD and BBC3. Isoform Bcl-X(L) binds to Siva isoform 1. Interacts with BCL2L11 (By similarity). Interacts with BECN1 and PGAM5. SUBCELLULAR LOCATION: Mitochondrion membrane; Single-pass membrane protein (By similarity). Nucleus envelope; Single-pass membrane protein; Cytoplasmic side (By similarity). Note=Mitochondrial membranes and perinuclear envelope (By similarity). TISSUE SPECIFICITY: Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain. DOMAIN: SwissProt: Q07817 The BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death. PTM: Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity. SIMILARITY: Belongs to the Bcl-2 family. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Store at 2-8°C for up to 12 months. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |