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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Ca, H, Ht, M, Po, R, Fe | FC, WB, ICC, IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB3226 |
| Replaces | MABT150 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Cytokeratin 7 Antibody, clone RCK105 |
| Background Information | Keratin, type II cytoskeletal 7 (UniProt: P08729; also known as Cytokeratin-7, CK-7, Keratin-7, K7, Sarcolectin, Type-II keratin Kb7) is encoded by the KRT7 (also known as SCL) gene (Gene ID: 3855) in human. Cytokeratins are the intermediate filament proteins of epithelia that display high degree of molecular diversity. They are heteropolymeric filaments formed by pairing of type I and type II keratins that are expressed in a highly specific patterns depending on the epithelial type and stage of cellular differentiation. They contain a central rod domain of about 310 amino acids with α-helical conformation bordered by non-helical head and tail domains of variable length. The head domain consists of subdomains V1 and H1. The central a-helical rod domain is composed of subdomains 1A, 1B, 2A, and 2B connected by the linkers L1, L12, and L2. The tail domain then consists of subdomains H2 and V2. They provide stability between epithelial cells and their attachment to basement membrane. Keratins also serve as tumor markers and epithelial differentiation markers. Multiple human epithelial keratins have been resolved with 2D-gels electrophoresis. These can be divided into acidic (pI <5.7) and basic (pI >6.0) subfamilies. The acidic keratins have molecular weights that range from 40 to 55 kDa. The basic keratins have molecular weights that range from 56 to 70 kDa. Members of the acidic and basic subfamilies are reported to be present in pairs. The composition of keratin pairs varies with the epithelial cell type, stage of differentiation, cellular growth environment, and the disease state. Keratin 7 is expressed in cultured epidermal, bronchial, and mesothelial cells. It is observed throughout the glandular cells in the junction between stomach and esophagus but is absent in esophagus. Its expression is also observed in a variety of carcinomas and in tumor cells of mammary and extramammary Paget disease. Keratin-7 expression can be used to distinguish ovarian and gastrointestinal carcinomas. (Ref.: Chu, P., et al. (2000). Modern Pathol. 13(9); 962-972; Kaba, A., et al. (1999). Biochimie. 81(7); 709-715). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Liquid in buffer with 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Cytokeratin 7 Antibody, clone RCK105 is an antibody against Cytokeratin 7 for use in FC, WB, IC, IH. |
| Key Applications |
|
| Application Notes | Western blot: 57-58kDa band in reduce westerns Immunohistochemistry on frozen tissue sections. Immunocytochemistry Flow cytometry Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Cytokeratins from the human bladder carcinoma cell line T24. |
| Clone | RCK105 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | MAB3226 reacts with a subgroup of glandular epithelial and their tumors, as well as transitional epithelium and transitional carcinoma. It reacts with a 58 kDa protein corresponding to cytokeratin 7. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P08729 # Plays a significant role in maintaining keratin filament organization in epithelia. SIZE: 469 amino acids; 51,386 Da SUBUNIT: Heterotetramer of two type I and two type II keratins. Associates with KRT8. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Expressed predominantly in the epithelium and in carcinomas. PTM: Arg-20 is dimethylated, probably to asymmetric dimethylarginine. SIMILARITY: SwissProt: P08729 Belongs to the intermediate filament family. MISCELLANEOUS: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa). |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots up to 6 months. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |