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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Ca, H, M, R | FC, WB, ICC, IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB3230 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Cytokeratin 10 Antibody, clone RKSE60 |
| Background Information | Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight from between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8, The individual cytokeratin polypeptides are designated 1-20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Liquid in buffer with 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Cytokeratin 10 Antibody, clone RKSE60 detects level of Cytokeratin 10 & has been published & validated for use in FC, WB, IC, IH. |
| Key Applications |
|
| Application Notes | Western blot Immunohistochemistry on frozen tissue sections. Immunocytochemistry Flow cytometry Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Cytoskeletal preparation from human squamous keratinizing epithelium. |
| Clone | RKSE60 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Cytokeratin 10. MAB3230 reacts with keratinizing stratified epithelia and in differentiated areas of highly differentiated squamous cell carcinomas. For the recognition of keratinizing cells in squamous cell carcinomas from epidermis, lung, bladder, cervix, esophagus etc. The antibody reacts only with suprabasal cells of the epidermis and not with basal cells. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | SIZE: 593 amino acids; 59511 Da SUBUNIT: Heterotetramer of two type I and two type II keratins. keratin-10 is generally associated with keratin-1. TISSUE SPECIFICITY: Seen in all suprabasal cell layers including stratum corneum. DISEASE: SwissProt: P13645 # Defects in KRT10 are a cause of epidermolytic hyperkeratosis (EHK) [MIM:113800]; also known as bullous congenital ichthyosiform erythroderma (BCIE) or bullous erythroderma ichthyosiformis congenita of Brocq. EHK is an hereditary skin disorder characterized by blistering and a marked thickening of the stratum corneum. At birth, affected individuals usually present with redness, blisters and superficial erosions due to cytolysis. Within a few weeks, the erythroderma and blister formation diminish and hyperkeratoses develop. Transmission is autosomal dominant, but most cases are sporadic. & Defects in KRT10 are a cause of annular epidermolytic ichthyosis (AEI) [MIM:607602]; also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI resembles clinical and histologic features of both epidermolytic hyperkeratosis and ichthyosis bullosa of Siemens. & Defects in KRT10 are a cause of epidermal nevus epidermolytic hyperkeratotic type [MIM:600648]. Epidermal nevi affect about 1 in 1,000 people. They appear at or shortly after birth as localized lines of epidermal thickening. The extent of skin involvement varies widely. SIMILARITY: SwissProt: P13645 ## Belongs to the intermediate filament family. MISCELLANEOUS: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa). |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots up to 6 months. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |