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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ELISA, WB, ICC, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB324 |
| Replaces | CBL220 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Neuron Specific Enolase Antibody, clone 5E2 |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Liquid in PBS pH 7.4 containing 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Neuron Specific Enolase Antibody, clone 5E2 detects level of Neuron Specific Enolase & has been published & validated for use in ELISA, WB, IC, IH(P). |
| Key Applications |
|
| Application Notes | Immunoblotting ELISA Immunohistochemistry. May be used on formalin-fixed, paraffin embedded sections. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Neuron-specific enolase from human brain. |
| Clone | 5E2 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Human gamma neuron specific enolase. Recognizes a 45kD band corresponding to gamma NSE on immunoblots of human brain extracts. |
| Isotype | IgG2a |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P09104 # Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity). COFACTOR: Magnesium. Required for catalysis and for stabilizing the dimer. SIZE: 434 amino acids; 47269 Da SUBUNIT: Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific. SUBCELLULAR LOCATION: Cytoplasm (By similarity). Cell membrane (By similarity). Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form (By similarity). TISSUE SPECIFICITY: The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.DEVELOPMENTAL STAGE: During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells. SIMILARITY: SwissProt: P09104 ## Belongs to the enolase family. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at 2-8 °C for up to 12 months under sterile conditions. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |