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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Ch, H, M, Mk, R, Fe | IP, ICC, IHC, IH(P) | M | Ascites | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB328 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Oligodendrocytes Antibody, clone CE-1 |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Ascites |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Oligodendrocytes Antibody, clone CE-1 is validated for use in IP, IC, IH, IH(P) for the detection of Oligodendrocytes. |
| Key Applications |
|
| Applications Not Recommended |
|
| Application Notes | Immunocytochemistry Immunohistochemistry at 1:1,000-1:5000 Not suggested for use in Western blot. Optimal working dilutions must be determined by end user. IMMUNOHISTOCHEMISTRY PROTOCOL FOR MAB328 This antibody has been used successfully on 30 mm, free floating, 4% paraformaldehyde fixed rat brain tissue. All steps are performed under constant agitation. Suggested protocol follows. 1) 3 x 10 minute washes in TBS (with or without 0.25% Triton). 2) Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody). 3) Incubate primary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (with or without 0.25% Triton) for 2 hours at room temperature followed by 16 hours at 4°C. 4) 3 x 10 minute washes in TBS. 5) Incubate with secondary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody). 6) 3 x 10 minute washes in TBS. 7) ABC Elite (1:200 Vector Labs) in TBS. 8) 2 x 10 minute washes in TBS. 9) 1 x 10 minute wash in phosphate buffer (no saline). 10) DAB reaction with 0.06% NiCl added for intensification. 11) 2 x 10 minute washes in PBS. 12) 1 x 10 minute wash in phosphate buffer (no saline). |
| Biological Information | |
|---|---|
| Immunogen | Rat glial membranes and whole brain white matter. |
| Clone | CE-1 |
| Host | Mouse |
| Specificity | In neonatal rat glia MAB328 stains oligodendrocytes and does not react with type 1 astrocytes, type 2 astrocytes or fibroblasts. Does not stain cultures of rat Schwann cells. In tissue sections MAB328 labels oligodendrocytes and CNS myelin with no labeling of peripheral myelin. MAB328 detects MOSP which is expressed at day 4-5 of neonatal rat, which is about 1-2 after the appearance of GC or sulfatide. The initial expression of MOSP occurs at the stage in development when oligodendrocytes are elaborating processes and just beginning to form membrane sheets (Mu QQ & C Dyer, 1994). |
| Isotype | IgM |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |