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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Rb | ELISA, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB3303 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Collagen Type VI Antibody, clone VI-26 |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Liquid in 0.1 M sodium phosphate buffer, pH 7.0, containing 2% protease-free bovine Serum albumin. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Collagen Type VI Antibody, clone VI-26 detects level of Collagen Type VI & has been published & validated for use in ELISA, IH(P). |
| Key Applications |
|
| Application Notes | Immunohistochemistry on acetone fixed paraffin-embedded tissues. Formalin fixation is not recommended. Antigen recovery is treatment with 0.04% trypsin in 0.01M CaCl(2) 0.05M Tris-HCL pH 7.6, 37°C for 10 minutes before the non-specific peroxidase block. EIA Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Clone | VI-26 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Specifically reacts with the hCL(VI). This is a purified mouse monoclonal antibody to human type VI collagen. VI-26 does not react with denatured or reduced type VI collagen, although this antibody does recognize the the triple helix consisting of alpha1(VI), alpha 2(VI) alpha3(gamma). The epitope has not been mapped and the monoclonal is know not to react with collagens I, II, III, IV or V in native assays. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes the major type VI alpha collagen chain of basement membranes. Like the other members of the type VI collagen gene family, this gene is organized in a head-to-head conformation with another type VI collagen gene so that each gene pair shares a common promoter. |
| Gene Symbol |
|
| Non-Reactive Species |
|
| Purification Method | Protein A Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: Collagen VI is a major structural component of microfibrils. The basic structural unit of collagen VI is a heterotrimer of the alpha1(VI), alpha2(VI), and alpha3(VI) chains. The alpha2(VI) and alpha3(VI) chains are encoded by the COL6A2 and COL6A3 genes, respectively. Type VI collagen is a unique beaded filament collagen. It is found in the interface between the basement membrane and interstitial matrix, where it forms a unique microfibrillar network. It has several binding partners. SIZE: 1028 amino acids; 108,529 Da SUBUNIT: Collagen VI is a heterotrimer of the alpha1(VI), alpha2(VI), and alpha3(VI) chains. SUBCELLULAR LOCATION: Extracellular matrix. TISSUE SPECIFICITY: Expressed in tendon of biceps brachii and many other tissues. PTM: Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. DISEASE: Mutations in COL6A1 gene have been linked to Bethlem myopathy that is characterized by early childhood onset and joint contractures most frequently affecting the elbows and ankles. |
| Product Usage Statements | |
|---|---|
| Availability by Geography |
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| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |