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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ELISA, WB, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB3319 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-MMP-14 Antibody, catalytic domain, clone 114-6G6 |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Liquid in 0.1 M sodium phosphate buffer, pH 7.0 containing 2% protease free bovine serum albumin. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-MMP-14 Antibody, catalytic domain, clone 114-6G6 is validated for use in ELISA, WB, IH(P) for the detection of MMP-14. |
| Key Applications |
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| Application Notes | Immunoblotting: 8-20 μg/mL. 66+60kDa (Ueno et al., 1997) Immunohistochemistry on paraffin-embedded tissue sections, use at 20 μg/mL concentration (see Ueno et al., 1997; Nakamura et al., 1999). Requires periodate-lysine-paraformaldehyde fixative (Namura et al., 1999) for 18-24 hrs. at 4°C. EIA Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Epitope | catalytic domain |
| Clone | 114-6G6 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | The antibody specifically reacts with human MT1-MMP. This is a purified mouse monoclonal antibody to an oligopeptide of REVPYAYIREGHEK (residue 160-173) on human membrane-type 1 matrix metalloproteinase (human MT1-MMP) and does not react with mouse or rat. However, this sequence is identical in mouse and rat MT1-MMP. For unknown reasons this antibody does not react on westerns to CC1043. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion. |
| Gene Symbol |
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| Non-Reactive Species |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P50281 # Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. COFACTOR: Binds 1 zinc ion per subunit (By similarity). & Calcium (By similarity). SIZE: 582 amino acids; 65884 Da SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein (Potential). Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. TISSUE SPECIFICITY: In stromal cells of colon, breast, and head and neck. DOMAIN: SwissProt: P50281 The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. SIMILARITY: Belongs to the peptidase M10A family. & Contains 4 hemopexin-like domains. |
| Product Usage Statements | |
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| Availability by Geography |
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| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Maintain frozen at -20°C in undiluted aliquots for up to 12 months. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |