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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Av, B, H, Po | IHC, IP, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB339 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-GABA A Receptor α1 chain Antibody, NT, clone BD24 |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Liquid. Buffer = 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-GABA A Receptor α1 chain Antibody, N-terminus, clone BD24 is an antibody against GABA A Receptor α1 chain for use in IH, IP & WB. |
| Key Applications |
|
| Application Notes | Immunohistochemistry: 10-20 μg/mL * See protocol below. Western blot: 20 μg/mL Immunoprecipitation Optimal working dilutions must be determined by end user. APPLICATION NOTES FOR MAB339 IMMUNOHISTOCHEMISTRY 1) Fresh tissue (human) should be used (3-6 hours postmortem). The tissue should be immersion fixed in 2% paraformaldehyde/0.1% glutaraldehyde. Prepare 50 mm sections and store in cryoprotective solution at -15°C. 2) Sections should be incubated floating in suitable small vials. 3) Block endogenous peroxidase with 100 mmol/L Tris-HCl, 150 mmol/L NaCl, pH 7.4 (Buffer A) containing 3% H2O2 (v/v) and 10% methanol for 20 min at room temperature. 4) Wash sections in Buffer A. Incubate sections in Buffer A containing 5% fetal bovine serum (v/v) and 0.1-0.5% Triton X-100 (v/v). 5) Wash sections in Buffer A. Incubate sections with MAB339 (diluted 10-20 mg/mL in Buffer A containing 5% fetal bovine serum (v/v) and 0.1-0.5% Triton X-100 (v/v) for 12-36 hours at +4°C 6) Wash sections in Buffer A. 7) Detect with standard secondary antibody detection system (PAP, ABC, etc.). 8) Wash sections in Buffer A. 9) Mount sections on chrome alum-coated slides, dry, dehydrate, and apply coverslips. |
| Biological Information | |
|---|---|
| Immunogen | Purified GABA/benzodiazepine receptor from bovine cortex. |
| Epitope | N-terminus |
| Clone | BD24 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Reacts with the alpha 1-chain of the GABAA receptor in bovine and human but not rat |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. |
| Gene Symbol |
|
| Non-Reactive Species |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P14867 # GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. SIZE: 456 amino acids; 51802 Da SUBUNIT: Binds UBQLN1 (By similarity). Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho. SUBCELLULAR LOCATION: Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. DISEASE: SwissProt: P14867 # Defects in GABRA1 are a cause of juvenile myoclonic epilepsy (JME) [MIM:606904]. JME is a common epileptic syndrome characterized by afebrile seizures, onset in adolescence (rather than in childhood) and myoclonic jerks. & Defects in GABRA1 are the cause of childhood absence epilepsy type 4 (ECA4) [MIM:611136]. ECA4 is a subtype of idiopathic generalized epilepsy (IGE) characterized by onset at age 6-7 years, frequent absence seizures (several per day) and bilateral, synchronous, symmetric 3-Hz spike waves on EEG. During adolescence, tonic-clonic and myoclonic seizures develop. Absence seizures may either remit or persist into adulthood. SIMILARITY: SwissProt: P14867 ## Belongs to the ligand-gated ionic channel (TC 1.A.9) family. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain lyophilized material at +2-8°C for up to 12 months. After reconstitution maintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |