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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| A, H, M, R | ICC, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB3408 |
| Replaces | 04-1049 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Tubulin Antibody, beta, clone KMX-1 |
| Alternate Names |
|
| Background Information | Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 kDa. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells. For example, expression of tubulin in adipose tissue is very low and therefore beta Tubulin should not be used as loading control for these tissues. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG2b liquid in buffer containing 0.02M phosphate buffer, 0.25M NaCl with 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Tubulin Antibody, beta, clone KMX-1 is validated for use in IC, WB for the detection of Tubulin. |
| Key Applications |
|
| Application Notes | Immunocytochemistry: A 1-2 μg/mL concentration of a previous lot was used in immunocytochemistry. Western blot: 0.05-0.5 μg/mL Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Tubulin purified from polycephalum myxamoebae |
| Clone | KMX-1 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody reacts specifically with β-tubulin and shows a strong cross-reactivity to different species. This antibody allows staining of microtubuli using indirect immunofluorescence showing a low background only. |
| Isotype | IgG2b |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein A Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: Q13509 # Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. SIZE: 450 amino acids; 50433 Da SUBUNIT: Dimer of alpha and beta chains. DOMAIN: SwissProt: Q13509 The highly acidic C-terminal region may bind cations such as calcium. SIMILARITY: Belongs to the tubulin family. |
| Molecular Weight | 50 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Western Blot on A431 lysates. Western blot: 1:500 dilution of this lot detected TUBULIN (BETA) on 10 μg of A431 lysates. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 6 months at 2-8ºC in undiluted aliquots from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |