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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, Ch, H, M, Qu, R, Xn | ICC | M | AlexaFluor®555 | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB3418A5 |
| Description | Anti-MAP2 Antibody, clone AP20, Alexa Fluor® 555 Conjugate |
| Alternate Names |
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| Background Information | The microtubule-associated protein 2 (MAP-2) is an abundant neuronal cytoskeletal protein that binds to tubulin, stabilizes microtubules (Herzog and Weber, 1978), and is an excellent marker for neurons. MAP-2 is essential for the development and maintenance of neuronal morphology (Matus, 1991). Three primary isoforms of MAP-2, the high molecular weight MAP2a and MAP2b, and the low molecular weight MAP2c (resulting from alternate splicing of the MAP2 gene), have been identified in neurons. (Chung et Al., 1996). The low molecular weight isoform, MAP2c, is expressed in developing brain and is down-regulated during brain maturation, whereas the high molecular weight MAP2b is expressed in both developing and adult brain. The MAP2a appears only after brain maturation (Tucker, 1990). All these forms bind to microtubules through a domain near the carboxyl terminus that contains either three or four similar repeats of a 31-amino-acid motif (Lewis et Al., 1988). MAP-2 together with MAP-4 and tau proteins belong to the family of thermostable proteins associated with microtubules. |
| Product Information | |
|---|---|
| Format | AlexaFluor®555 |
| Control |
|
| Presentation | Purified mouse monoclonal IgG conjugated to Alexa Fluor® 555 in PBS with 0.1% sodium azide and 15 mg/mL BSA. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-MAP2 Antibody, clone AP20, Alexa Fluor 555 Conjugate is validated for use in ICC for the detection of MAP2. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | Bovine brain microtubule protein. |
| Clone | AP20 |
| Host | Mouse |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Bovine, Chicken, Human, Mouse, Quail, Rat, Xenopus. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isforms have been described. |
| Gene Symbol |
|
| Purification Method | Affinity purfied. |
| UniProt Number |
|
| UniProt Summary | Function The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. Subcellular location Cytoplasm › cytoskeleton Probable. Post-translational modification Phosphorylated at serine residues in K-X-g-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly By similarity. MAP2A/c is phosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR. Isoform MAP2c is phosphorylated by FYN at Tyr-67. Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Sequence similarities Contains 3 Tau/MAP repeats. |
| Molecular Weight | ~230 kDa observed. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunocytochemistry in rat E18 primary cortex cells. Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected MAP2 in rat E18 primary cortex cells. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |