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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R, B, Ch | ICC | M | Biotin | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB3418B |
| Description | Anti-MAP2 Antibody, clone AP20, Biotin Conjugated |
| Alternate Names |
|
| Background Information | The microtubule-associated protein 2 (MAP-2) is an abundant neuronal cytoskeletal protein that binds to tubulin, stabilizes microtubules (Herzog and Weber, 1978), and is an excellent marker for neurons. MAP-2 is essential for the development and maintenance of neuronal morphology (Matus, 1991). Three primary isoforms of MAP-2, the high molecular weight MAP2a and MAP2b, and the low molecular weight MAP2c (resulting from alternate splicing of the MAP2 gene), have been identified in neurours. (Chung et Al., 1996). The low molecular weight isoform, MAP2c, is expressed in developing brain and is down-regulated during brain maturation, whereas the high molecular weight MAP2b is expressed in both developing and adult brain. The MAP2a appears only after brain maturation (Tucker, 1990). All these forms bind to microtubules through a domain near the carboxyl terminus that contains either three or four similar repeats of a 31-amino-acid motif (Lewis et Al., 1988). MAP-2 together with MAP-4 and tau proteins belong to the family of thermostable proteins associated with microtubules. |
| Product Information | |
|---|---|
| Format | Biotin |
| Control |
|
| Presentation | Purified mouse monoclonal IgG conjugated to Biotin in PBS with 0.1% sodium azide and 15 mg/mL BSA. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-MAP2 Antibody, clone AP20, Biotin Conjugated is an antibody against MAP2 for use in ICC. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:25 dilution from a representative lot detected MAP2 in rat E18 primary cortex cells. |
| Biological Information | |
|---|---|
| Immunogen | Bovine brain microtubule protein |
| Clone | AP20 |
| Host | Mouse |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Rat. Predicted to react with Chicken, Human, and Mouse based on 100% sequence homology. Predicted to react with Bovine based on immunogen design. |
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
|
| Purification Method | Protein g Purified |
| UniProt Number |
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| Molecular Weight | The unconjugated parent antibody (Cat. No. MAB3418) has an observed molecular weight at ~230 kDa. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in rat cortex tissue. Immunohistochemistry Analysis: A 1:25 dilution of this antibody detected MAP2 in rat cortex tissue. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain refrigerated at 2-8 °C in undiluted aliquots for up to 6 months from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |