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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Ma, H, B, R, Xn, Op, Ht | ICC, IHC, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB3422 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Histone Antibody, clone H11-4 |
| Background Information | There are 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. The five major classes of histones are H1/H5, H2A, H2B, H3, and H4. Histones H2A, H2B, H3 and H4 are known as the core histones, while histones H1 and H5 are known as the linker histones. Histones feature a main globular domain and a long N terminal tail which are involved with the structure of the nucleosomes of the beads on a string structure. They are the chief protein components of chromatin, acting as spools around which DNA winds, and play a role in gene regulation. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG1 in buffer containing 0.02M phosphate buffer, pH 7.6 with 0.25M NaCl and 0.1% sodium azide |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Histone with Anti-Histone Antibody, clone H11-4 (Mouse Monoclonal Antibody), that has been shown to work in ICC, IHC & WB. |
| Key Applications |
|
| Application Notes | Western Blot Analysis: 1:500 dilution of a previous lot was used. Immunocytochemistry: A 1-5 µg/mL concentration of a previous lot was used on Immunocytochemistry. Immunohistochemistry: A 1-5 μg/mL concentration of a previous lot was used on Immunohistochemistry. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Purified nuclear fractions of HeLa cells |
| Clone | H11-4 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | The antibody binds specifically to an antigen determinant, which is present on all five histone proteins. The antibody recognizes the histones H1, H2A, H2B, H3 and H4 with comparable sensitivity. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Species Reactivity Note | Due to the highly conserved nature of histones, this antibody is predicted to have wide species reactivity. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. |
| Purification Method | Protein A Purfied |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Western Blot on Jurkat lysates. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 6 months at 2-8ºC in undiluted aliquots from date of receipt. The Iyophilized antibody is stable at 2–8°C. Store the reconstituted antibody in aliquots at -15 to -25°C for up to 12 months.Repeated freezing and thawing should be avoided. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |