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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, Fe, Ht | IHC, IP, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB347 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Growth Associated Protein 43 Antibody, clone 9-1E12 |
| Alternate Names |
|
| Background Information | GAP-43, a common marker of differentiating neurons, is expressed at elevated levels by developing or regenerating neurons during axon growth. While GAP-43 is an integral membrane protein associated with the cytoplasmic surface of axonal growth cones (and synapses), it is absent from dendritic growth cones. In adult brain, GAP-43 is found in high concentration in presynaptic areas where memory formation is thought to occur, such as frontal cortex, limbic system and hippocampus. Phosphorylation of GAP-43 by PKC (at Ser41 on human, mouse, rat, and bovine GAP-43 or Ser42 on chicken and Xenopus GAP-43) is specifically correlated with certain forms of synaptic plasticity. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Protein A Purified mouse immunoglobulin IgG1 in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Growth Associated Protein 43 Antibody, clone 9-1E12 detects level of Growth Associated Protein 43 & has been published & validated for use in IH, IP & WB. |
| Key Applications |
|
| Application Notes | Immunohistochemistry: 0.1-0.5 µg/mL of a previous lot on 4% paraformaldehyde perfused and fixed rat cerebellum and cerebrum tissue. The addition of 0.01-0.1% Triton X-100 to incubation buffer will increase cellular permeability. Immunoprecipitation: A previous lot of this antibody was used in immunoprecipitation. 5-10µg/mL in membrane preparations from 16-18 day embryonic cortical neuronal cultures. Western Blot Analysis: MAB347 will detect a single 43-48 kD band in western blots of membrane fractions of growing neurons. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | GAP-43 purified from rat brain |
| Clone | 9-1E12 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | GAP-43 (also known as growth associated protein-43, B-50, F1 and pp46), regardless of the protein’s phosphorylation state. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Species Reactivity Note | Rat, feline and hamster. Expected to react with human based on sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene has been termed a growth or plasticity protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This encoded protein is considered a crucial component of an effective regenerative response in the nervous system. |
| Gene Symbol |
|
| Purification Method | Protein A Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P17677 # This protein is associated with nerve growth. It is a major component of the motile growth cones that form the tips of elongating axons. SIZE: 238 amino acids; 24803 Da SUBUNIT: Binds calmodulin with a greater affinity in the absence of Ca(2+) than in its presence (By similarity). SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, growth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, synapse. Note=Cytoplasmic surface of growth cone and synaptic plasma membranes. PTM: Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity. SIMILARITY: SwissProt: P17677 ## Belongs to the neuromodulin family. & Contains 1 IQ domain. |
| Molecular Weight | 43-48 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Western Blot on rat brain lysates. Western Blot Analysis: 1:1000 dilution of this lot detected GAP-43/B-50 on 10 μg of rat brain lysates. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at 2-8°C in undiluted for up to 6 months after date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |