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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | ICC, IP | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB3494 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-ATP Synthase Antibody, β chain, clone 4.3E8.D1 |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-ATP Synthase Antibody, β chain, clone 4.3E8.D1 detects level of ATP Synthase & has been published & validated for use in IC & IP. |
| Key Applications |
|
| Application Notes | Immunocytochemistry: 1 μg/mL for one hour at room temperature using 10xE6 cells or adherent sample by indirect immunofluorescence. Suggested fixative is 2-4% paraformaldehyde. Suggested permeabilization method is 0.5% Triton X-100. Immunoprecipitation Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Rat brain mitochondria |
| Epitope | beta chain |
| Clone | 4.3E8.D1 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Recognizes the beta chain of ATP Synthase. The beta chain of ATP Synthase is a useful subcellular marker for mitochondria. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P06576 # Produces ATP from ADP in the presence of a proton gradient across the membrane. The beta chain is the catalytic subunit. SIZE: 529 amino acids; 56560 Da SUBUNIT: F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c. SUBCELLULAR LOCATION: Mitochondrion. SIMILARITY: SwissProt: P06576 ## Belongs to the ATPase alpha/beta chains family. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |