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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Ca, H, M, Mk, R | ELISA, FC, IP, WB | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB3501 |
| Replaces | 04-571 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Caspase 2 Antibody, clone 10C6 |
| Alternate Names |
|
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal antibody in buffer containing PBS, 0.1% Kathon. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Caspase 2 Antibody, clone 10C6 detects level of Caspase 2 & has been published & validated for use in ELISA, FC, IP & WB. |
| Key Applications |
|
| Application Notes | Western blot: Recommended usage at 5 μg/ml, which has been found to detect endogenous Caspase-2 in 5 x 10E5 HeLa and MCF-7 cells. Immunoprecipitation: Recommended concentration 1-5 μg/ml FACS: Recommended concentration 1 μg/ml. It is necessary to permeabilize the cell membrane prior to analysis. EIA: Recommended concentration 0.5-1 μg/ml. Immunohistochemistry: Use at 10-20 μg/ml. It is necessary to fix the cells in 4% Paraformaldehyde/0.18% Triton-X100 for 10 minutes prior to staining. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Raised against the P19 subunit of recombinant human Caspase-2 protein. |
| Clone | 10C6 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rat |
| Specificity | MAB3501 antibody recognizes a reactive epitope that lies in the P19 subunit of human Caspase-2 (ICH-1, Nedd-2). |
| Isotype | IgG2a |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The proteolytic cleavage of this protein is induced by a variety of apoptotic stimuli. Alternative splicing of this gene results in multiple transcript variants that encode different isoforms. |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P42575 # Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival. SIZE: 452 amino acids; 50685 Da SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a p18 subunit and a p12 subunit. Interacts with LRDD. TISSUE SPECIFICITY: Expressed in larger amounts in the embryonic lung, liver and kidney than in the heart and brain. In the adults higher level expression is seen in the placenta, lung, kidney, pancreas than in the heart, brain, liver and skeletal muscle. PTM: The mature protease can process its own propeptide, but not that of other caspases. SIMILARITY: SwissProt: P42575 ## Belongs to the peptidase C14 family. & Contains 1 CARD domain. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |