Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IHC, IH(P), WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB3564 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Sodium/Iodine Symporter Antibody, a.a. 625-643, clone FP5A |
| Alternate Names |
|
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Liquid. Buffer = 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Sodium/Iodine Symporter using this Anti-Sodium/Iodine Symporter Antibody, a.a. 625-643, clone FP5A validated for use in IH, IH(P) & WB. |
| Key Applications |
|
| Application Notes | Immunoblotting. The antibody recognizes a major band at ~97 kDa and minor bands at 15 kDa, 30 kDa, 68 kDa and 160 kDa. Immunohistochemistry. The antibody works on paraffin embedded tissue sections which have been subjected to antigen retrieval with 10 mM citrate buffer steam for 30 minutes. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Maltose Binding Protein fusion protein corresponding to amino acids 468-643 of human NIS (C-terminus). |
| Epitope | a.a. 625-643 |
| Clone | FP5A |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Sodium/Iodide Symporter (NIS). The epitope is thought to be in the region of amino acids 625-643 of human NIS. The antibody may show some reactivity to Maltose Binding Protein. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The sodium-iodide symporter (NIS), a member of the sodium/solute symporter family, actively transports iodide across the basolateral plasma membrane of the thyroid follicular cells.[supplied by OMIM] |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: Q92911 # Mediates iodide uptake in the thyroid gland. SIZE: 643 amino acids; 68666 Da SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Expression is primarily in thyroid tissue, but also to a lower extent in mammary gland and ovary. Expression is reduced in tumors. DISEASE: SwissProt: Q92911 # Defects in SLC5A5 are the cause of iodide transport defect (ITD) [MIM:274400]. ITD is characterized by an inability of the thyroid to maintain a concentration difference of readily exchangeable iodine between the plasma and the thyroid gland. It is a cause of congenital hypothyroidism. SIMILARITY: SwissProt: Q92911 ## Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at 2-8°C in undiluted aliquots for up to 6 months. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |