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Millipore Detect Green Fluorescent Protein Using This Anti-Green Fluorescent Protein Antibody Validated For Use In Elisa, Ic, Ih & Wb. - Mill (Additional S&H or Hazmat Fees May Apply)
The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.
Product Information
Format
Purified
Control
GFP fusion protein expressed in cells
Presentation
Liquid in PBS containing 1 mg/mL BSA. Contains no preservative.
Quality Level
MQ100
Applications
Application
Detect Green Fluorescent Protein using this Anti-Green Fluorescent Protein Antibody validated for use in ELISA, IC, IH & WB.
Key Applications
ELISA
Immunocytochemistry
Immunohistochemistry
Western Blotting
Application Notes
Western blot: 1:2,500-1:5,000. Recommended dilution buffer is PBS containing 1 mg/mL BSA.
Immunocytochemistry: 1:500-1:1,000
Immunohistochemistry: 1:500-1:1,000 on paraformaldehyde fixed tissues with an overnight incubation using Nickel enhanced DAB for detection. Suggested permeabilization method is 0.5% Triton X-100. Recommended blocking buffer is PBS containing BSA and serum from the host of the secondary antibody. Recommended dilution buffer is PBS containing 1 mg/mL of BSA.
ELISA: 1:1,000-1:2,000 in a direct assay.
Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen
GFP-BSA
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
Reacts with Green Fluorescent Protein (GFP). The antibody reacts with various forms of GFP including eGFP including those found in vectors supplied by Clonetech and Invitrogen. There is not cross-reactivity to RFP. YFP has not been tested. This antibody works well for immunohistochemistry.
Isotype
IgG1
Species Reactivity
All
Antibody Type
Monoclonal Antibody
Purification Method
Protein A Purfied
UniProt Number
P42212
Molecular Weight
MW for GFP is 27 kDa. Dependent upon the molecular weight of the fusion protein being detected.
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
