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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, ICC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB3612 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-BubR1 Antibody, clone 8G1 |
| Background Information | The mitotic checkpoint prevents the resulting two daughter cells from having unequal numbers of chromosomes. This condition, known as aneuploidy, is present in almost all cancer cells and may be due to malfunctions in the mitotic checkpoint. BubR1 is a mitotic checkpoint kinase and has been implicated in the metaphase checkpoint control in mammalian cells. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Liquid |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect BubR1 with Anti-BubR1 Antibody, clone 8G1 (Mouse Monoclonal Antibody), that has been shown to work in WB, ICC. |
| Key Applications |
|
| Application Notes | Western blot using an ECL detection system. Reacts with the ~120 kDa BubR1 protein and higher bands due to hyperphosphorylations. Immunocytochemistry: 1 μg/mL on tissue culture cells. Suggested fix is 3.5% PBS-buffered paraformaldehyde for 7 minutes. Permeabilization method is 0.2% Triton-X-100 after fixation. Suggested blocking buffer is 10 mM Tris, pH 7.5 with 150 mM NaCl with 0.1% BSA. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant human BubR1. |
| Clone | 8G1 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Recognizes human BubR1. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer. |
| Gene Symbol |
|
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: O60566 # Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression. SIZE: 1050 amino acids; 119545 Da SUBUNIT: Interacts with CENPE, CENPF, mitosin and BUB3. Part of a complex containing BUB3, CDC20 and BUB1B. Interacts with anaphase- promoting complex/cyclosome (APC/C). SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Kinetochore. Note=Cytoplasmic in interphase cells. Associates with the kinetochores. TISSUE SPECIFICITY: Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index. DOMAIN: SwissProt: O60566 The CD1 domain directs kinetochore localization and binding to BUB3. & The D-box targets the protein for rapid degradation by ubiquitin-dependent proteolysis during the transition from mitosis to interphase (Potential). PTM: Proteolytically cleaved by caspase-3 in a cell cycle specific manner. The cleavage might be involved in the durability of the cell cycle delay. Caspase-3 cleavage is associated with abrogation of the mitotic checkpoint. The major site of cleavage is at Asp- 610. & Ubiquitinated (Probable). Degradated by the proteasome. & Autophosphorylated in vitro. Phosphorylated during mitosis and hyperphosphorylated in mitotically arrested cells. DISEASE: SwissProt: O60566 # Defects in BUB1B are associated with tumor formation. & Defects in BUB1B are the cause of premature chromatid separation trait (PCS) [MIM:176430]. PCS consists of separate and splayed chromatids with discernible centromeres and involves all or most chromosomes of a metaphase. It is found in up to 2% of metaphases in cultured lymphocytes from approximately 40% of normal individuals. When PCS is present in 5% or more of cells, it is known as the heterozygous PCS trait and has no obvious phenotypic effect, although some have reported decreased fertility. Inheritance is autosomal dominant. & Defects in BUB1B are the cause of mosaic variegated aneuploidy syndrome (MVA) [MIM:257300]. MVA is a severe autosomal recessive developmental disorder characterized by mosaic aneuploidies, predominantly trisomies and monosomies, involving multiple different chromosomes and tissues. The proportion of aneuploid cells varies but is usually more than 25% and is substantially greater than in normal individuals. Affected individuals typically present with severe intrauterine growth retardation and microcephaly. Eye anomalies, mild dysmorphism, variable developmental delay, and a broad spectrum of additional congenital abnormalities and medical conditions may also occur. The risk of malignancy is high, with rhabdomyosarcoma, Wilms tumor and leukemia reported in several cases. MVA is caused by biallelic mutations in the BUB1B gene. SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. BUB1 subfamily. & Contains 1 CD1 domain. & Contains 1 protein kinase domain. |
| Product Usage Statements | |
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| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |