Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, ICC, IHC | M | Ascites | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB362 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-MAP1 Antibody, clone HM-1 |
| Alternate Names |
|
| Product Information | |
|---|---|
| Format | Ascites |
| Presentation | Ascites fluid. Liquid containing 15mM sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect MAP1 using this Anti-MAP1 Antibody, clone HM-1 validated for use in WB, IC, IH. |
| Key Applications |
|
| Application Notes | Immunoblotting at 1:500 using fresh rat brain extract or enriched microtubule protein preparation. Immunocytochemistry Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Rat brain microtubule-associated protein. |
| Clone | HM-1 |
| Host | Mouse |
| Specificity | Reacts specifically with MAP1 (also known as MAP 1a) on immunoblots of whole brain homogenate proteins. Shows selective staining of neurons and stronger staining of axons and dendrites in immunohistochemistry staining of brain tissue. Stains both the cell bodies and dendritic trees. No cross-reactivity observed to other MAPs or tubulin. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The product of this gene is a precursor polypeptide that presumably undergoes proteolytic processing to generate the final MAP1A heavy chain and LC2 light chain. Expression of this gene is almost exclusively in the brain. Studies of the rat microtubule-associated protein 1A gene suggested a role in early events of spinal cord development. |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P78559 # Structural protein involved in the filamentous cross- bridging between microtubules and other skeletal elements. SIZE: 2805 amino acids; 306482 Da SUBUNIT: 3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins. Interacts with guanylate kinase- like domain of DLG1, DLG2 and DLG4 (By similarity). TISSUE SPECIFICITY: Brain. DOMAIN: SwissProt: P78559 The basic region containing the repeats may be responsible for the binding of MAP1A to microtubules. PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. & LC2 is generated from MAP1A by proteolytic processing. SIMILARITY: Belongs to the MAP1 family. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For antibodies with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the containers cap. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |