Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | ELISA, WB, ICC | M | Ascites | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB3632 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-PPAR gamma 1 and 2 Antibody, phospho-specific (Ser112) |
| Alternate Names |
|
| Product Information | |
|---|---|
| Format | Ascites |
| Presentation | Ascites. Liquid. Contains no preservative. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Use Anti-PPAR gamma 1 & 2 Antibody, phospho-specific (Ser112) (mouse monoclonal antibody) validated in ELISA, WB, ICC to detect PPAR gamma 1 & 2 also known as Peroxisome Proliferator Activated Receptor γ 1 & 2. |
| Key Applications |
|
| Application Notes | Western blot: 1:500-1:5,000 on endogenous and recombinant protein. Immunocytochemistry: 1:500-1:5,000 ELISA: 1:500-1:5,000 Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Phosphorylated (serine 112) synthetic peptide from amino acids 107-121 of human PPAR gamma2 (identical to PPAR gamma1). No homology with PPAR alpha or PPAR beta. |
| Epitope | phospho-specific (Ser112) |
| Host | Mouse |
| Specificity | Recognizes phosphorylated Peroxisome Proliferator Activated Receptor (PPAR) gamma1 and gamma2. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described. |
| Gene Symbol |
|
| Modifications |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P37231 # Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the receptor binds to a promoter element in the gene for acyl-CoA oxidase and activates its transcription. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. SIZE: 505 amino acids; 57620 Da SUBUNIT: Forms a heterodimer with the retinoic acid receptor RXRA called adipocyte-specific transcription factor ARF6. Interacts with NCOA6 coactivator, leading to a strong increase in transcription of target genes. Interacts with coactivator PPARBP, leading to a mild increase in transcription of target genes (By similarity). Interacts with NOCA7 in a ligand-inducible manner. Interacts with NCOA1 LXXLL motifs. Interacts with TGFB1I1. SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Highest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also are detectable in placenta, lung and ovary. DISEASE: SwissProt: P37231 # Defects in PPARG can lead to type 2 insulin-resistant diabetes and hyptertension. & Defects in PPARG may be associated with susceptibility to obesity [MIM:601665]. & Defects in PPARG may be associated with colon cancer. & Defects in PPARG are the cause of familial partial lipodystrophy type 3 (FPLD3) [MIM:604367]. Familial partial lipodystrophies (FPLD) are a heterogeneous group of genetic disorders characterized by marked loss of subcutaneous (sc) fat from the extremities. Affected individuals show an increased preponderance of insulin resistance, diabetes mellitus and dyslipidemia. & Variation in PPARG is associated with carotid intimal medial thickness 1 (CIMT1) [MIM:609338]. CIMT is a measure of atherosclerosis that is independently associated with traditional atherosclerotic cardiovascular disease risk factors and coronary atherosclerotic burden. 35 to 45% of the variability in multivariable-adjusted CIMT is explained by genetic factors. SIMILARITY: SwissProt: P37231 ## Belongs to the nuclear hormone receptor family. NR1 subfamily. & Contains 1 nuclear receptor DNA-binding domain. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |