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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Ch, H, M, Xn, F | WB, ICC, IHC | M | Ascites | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB3658 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-TATA-Binding-Protein Antibody |
| Alternate Names |
|
| Product Information | |
|---|---|
| Format | Ascites |
| Presentation | Ascites. Liquid. Contains no preservative. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-TATA-Binding-Protein Antibody is a Mouse Monoclonal Antibody for detection of TATA-Binding-Protein also known as TBP & has been validated in WB, ICC & IHC. |
| Key Applications |
|
| Application Notes | Western blot: 1:1,000-1:5,000 on endogenous and recombinant protein. Immunohistochemistry: 1:1,000-1:5,000 Immunocytochemistry: 1:1,000-1:5,000 Immunoprecipitation ELISA: 1:1,000-1:5,000 Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Full length recombinant human TBP. |
| Epitope | a.a. 1-18 |
| Host | Mouse |
| Specificity | Recognizes TATA-binding-protein (TBP). Recognizes an epitope from the first 18 amino acids of human TBP. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Species Reactivity Note | Human, mouse, chicken, xenopus and zebrafish. Does not react with Drososphila. Reactivity with other species has not been confirmed. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminal. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. Mutations that expand the number of CAG repeats encoding this polyglutamine tract, and thus increase the length of the polyglutamine string, are associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease. |
| Gene Symbol |
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| Non-Reactive Species |
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| Purification Method | Unpurified |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P20226 # General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre- initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. SIZE: 339 amino acids; 37698 Da SUBUNIT: Belongs to the TFIID complex together with the TBP- associated factors (TAFs). Component of the transcription factor SL1/TIFIB complex, composed of TBP and at least TAF1A, TAF1B TAF1C, and TAF3. Binds DNA as monomer. Interacts with TAFs, TFIIB, NCOA6, DRAP1, DR1 and ELF3. Interacts with SPIB, SNAPC1, SNAPC2 and SNAPC4. Interacts with HIV-1 Tat. Interacts with UTF1 which acts as a coactivator of ATF2 transcriptional activity. SUBCELLULAR LOCATION: Nucleus. DISEASE: SwissProt: P20226 # Defects in TBP are the cause of spinocerebellar ataxia 17 (SCA17) [MIM:607136]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of autosomal dominant cerebellar ataxias (ADCA). Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements. Spinocerebellar ataxia is caused by degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. The molecular defect in SCA17 is the expansion of a CAG repeat in the coding region of TBP. Longer expansions result in earlier onset and more severe clinical manifestations of the disease. SIMILARITY: SwissProt: P20226 ## Belongs to the TBP family. |
| Molecular Weight | ~42 kDa |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |