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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Ma, Mk, R | ICC, IHC, IP | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB367 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Muscarinic Acetylcholine Receptor m2 Antibody, clone M2-2-B3 |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified immunoglobulin. Liquid in 0.02 M phosphate buffer, 0.25 M NaCl with 0.1% sodium azide, pH 7.6. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Muscarinic Acetylcholine Receptor m2 Antibody, clone M2-2-B3 is an antibody against Muscarinic Acetylcholine Receptor m2 for use in IC, IH & IP. |
| Key Applications |
|
| Applications Not Recommended |
|
| Application Notes | Immunohistochemistry on 4% paraformaldehyde fixed tissue. Does not work on paraffin embedded tissues. Suggested starting concentration 1-5 μg/mL. It is suggested that you use the PAP system if using this antibody on rat. Immunocytochemistry on transfected cells Immunoprecipitation Works poorly for immunoblotting Optimal working dilutions must be determined by end user. IMMUNOHISTOCHEMISTRY PROTOCOL FOR MAB367 This antibody has been used successfully on 30 mm, free floating, 4% paraformaldehyde fixed rat brain tissue. All steps are performed under constant agitation. Suggested protocol follows. 1) 3 x 10 minute washes in TBS (with or without 0.25% Triton). 2) Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody). 3) Incubate primary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (with or without 0.25% Triton) for 2 hours at room temperature followed by 16 hours at 4°C. 4) 3 x 10 minute washes in TBS. 5) Incubate with secondary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody). 6) 3 x 10 minute washes in TBS. 7) ABC Elite (1:200 Vector Labs) in TBS. 8) 2 x 10 minute washes in TBS. 9) 1 x 10 minute wash in phosphate buffer (no saline). 10) DAB reaction with 0.06% NiCl added for intensification. 11) 2 x 10 minute washes in PBS. 12) 1 x 10 minute wash in phosphate buffer (no saline). |
| Biological Information | |
|---|---|
| Immunogen | i3 loop of m2 receptor fusion protein (225-359), fused to Glutathione S-transferase. |
| Clone | M2-2-B3 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rat |
| Specificity | m2 muscarinic acetylcholine receptor. No reactivity with the other subtypes. SPECIES REACTIVITIES: Expected to react with most mammalian species. |
| Isotype | IgG2a |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine to these receptors and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 2 is involved in mediation of bradycardia and a decrease in cardiac contractility. Multiple alternatively spliced transcript variants have been described for this gene. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P08172 # The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. SIZE: 466 amino acids; 51715 Da SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. SIMILARITY: SwissProt: P08172 ## Belongs to the G-protein coupled receptor 1 family. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at 2-8°C in undiluted aliquots for up to 6 months. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |