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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Ca, H, M, R, Rb, F, Fe, PO | ELISA, IP, ICC, IF, IHC, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB374 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody, clone 6C5 |
| Alternate Names |
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| Background Information | Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an ubiquitous glycolytic enzyme present in reasonably high levels in almost all tissues. As a house-keeping enzyme, it catalyzes the synthesis of 1,3-biphosphoglycerate, a "high energy" intermediate used for the synthesis of ATP. Besides its cytoplasmic action in metabolism it is also involved in the initial stages of apoptosis or oxidative stress response where GAPDH is translocated to the nucleus. Such actions may reflect the role of GAPDH in DNA repair or as one nuclear carrier for apoptotic molecules. GAPDH has also been found to bind specifically to proteins implicated in the pathogenesis of a variety of neurodegenerative disorders including the beta-amyloid precursor protein and the huntingtin protein where decreased function of GAPDH is associated with Alzheimers and Huntingtons disease fibroblasts. GAPDH has also been identified as a potential target for nitric oxide (NO)-mediated cellular toxicity. The complete and functional enzyme is a tetramer with each of four identical subunits occupying the vertex of a tetrahedron. Binding domains also include one principally interacting with NAD+ and another interacting with glyceraldehyde 3-phosphate (GAP). |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified Mouse monoclonal IgG1 in PBS, pH 7.4 with 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody, clone 6C5 is a well published and extensively characterized monoclonal antibody. This purified mAb detects Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) & has been published & validated for use in ELISA, IP, IC, IF, IH & WB. |
| Key Applications |
|
| Application Notes | ELISA: 1:1,000,000 dilution of a previous lot ws used for Immunoaffinity purification of GAPDH. Immunocytochemistry: 1:100 to 1:300 dilution of a previous lot was used in immunocytochemistry in PBS-BSA 10 mg/mL. 4% PFA, with 0.02% PBS-Triton X-100; 3 min RT (Griffoni, 2001). Western blot: 1:100 to 1:300. Recognizes a 36kDa band of the reduced monomer. Non-reduced GAPDH runs as a 146kDa tetramer. Immunoaffinity purification of GAPDH. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Glyceraldehyde-3-phosphate dehydrogenase from rabbit muscle. |
| Clone | 6C5 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from skeletal muscle. Antibody also recognizes cardiac GAPDH. GAPDH enzyme is detected in many non-muscle cells lines including HeLa, HCT-116 cells, U937 and THP-1 cells among others. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Species Reactivity Note | Human, porcine, canine, rat, mouse, rabbit, cat, and fish skeletal muscle. It has been reported that this antibody does not react with GAPDH from E. coli. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Glyceraldehyde-3-phosphate dehydrogenase catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. A GAPD pseudogene has been mapped to Xp21-p11 and 15 GAPD-like loci have been identified. |
| Gene Symbol |
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| Non-Reactive Species |
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| Purification Method | Protein A Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P04406 # Independent of its glycolytic activity it is also involved in membrane trafficking in the early secretory pathway. SIZE: 335 amino acids; 36053 Da SUBUNIT: Homotetramer. Binds PRKCI. SUBCELLULAR LOCATION: Cytoplasm. Note=Postnuclear and Perinuclear regions. Membrane. SIMILARITY: SwissProt: P04406 ## Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. |
| Molecular Weight | 38 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot on A431 lysates. Western Blot Analysis: 1:500 dilution of this antibody detected GLYCERALDEHYDE-3-PDH on 10 μg of A431 lysates. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at 2-8ºC in undiluted aliquots for up to 6 months after date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 200 µg |