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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IF, IP | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB3775 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Cdc20 Antibody, clone AR12 |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified immunoglobulin. Liquid in 0.02M phosphate buffer, 0.25M NaCl, pH 7.6, with 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Cdc20 Antibody, clone AR12 is a Mouse Monoclonal Antibody for detection of Cdc20 also known as Cell Division Cycle Protein 20 Homolog p55cdc & has been validated in IF & IP. |
| Key Applications |
|
| Application Notes | Immunofluorescence Immunoprecipitation Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Human Cdc20 recombinant protein |
| Clone | AR12 |
| Host | Mouse |
| Specificity | Recognizes human Cdc20 |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosome separation. |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: Q12834 # Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1. In metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. SIZE: 499 amino acids; 54723 Da SUBUNIT: Found in a complex with CDC20, CDC27, SPATC1 and TUBG1. Interacts with SPATC1 (By similarity). Interacts with MAD2L1. The phosphorylated form interacts with APC/C. DEVELOPMENTAL STAGE: Synthesis is initiated at G1/S, protein level peaks in M phase and protein is abruptly degraded at M/G1 transition. PTM: Phosphorylated during mitosis, probably by maturation promoting factor (MPF). SIMILARITY: SwissProt: Q12834 ## Belongs to the WD repeat CDC20/Fizzy family. & Contains 7 WD repeats. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |