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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IH(P), ICC, IF, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB3786-C |
| Description | Anti-APC Antibody, CT, clone C-APC Antibody 28.9, Ascites Free |
| Alternate Names |
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| Background Information | Adenomatous polyposis coli protein (UniProt P25054; also known as Deleted in polyposis 2.5, Protein APC) is encoded by the APC (also known as DP2.5) gene (Gene ID 324) in human. The multifunctional tumor suppressor APC is a large scaffold protein that contains multiple armadillo repeat (ARM) domains (a.a. 453-767 of human APC spliced isoform 1) for mediating various cellular functions, including Wnt signal regulation, cell adhesion, and chromosomal segregation during mitosis. APC, AXIN, casein kinase 1 (CK1) and glycogen synthase kinase 3 (GSK3) constitute the core proteins of the β-catenin destruction complex that functions as the negative regulator against Wnt signaling transduction. Mice with Apc gene mutations develop adenomas in the intestine due to Wnt hyperactivation, and functional loss of APC due to gene mutations is the hallmark of human colorectal cancers (CRC). APC also directs cholinergic synapse assembly between neurons and is commonly employed as a marker for monitoring stem cells neuronal differentiation. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-APC Antibody, CT, clone C-APC Antibody 28.9, Ascites Free is an antibody against APC for use in Immunohistochemistry (Paraffin), Immunocytochemistry, Immunofluorescence, Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected APC in human kidney and cardiac muscle. Immunocytochemistry Analysis: A representative detected an increased APC expression in human eyelid adipose-derived stem cells (hEASCs) after neuroinduction at passage 8 (Zhou, J., et al. (2014). J. Cell. Mol. Med. 18(2):326-343). Immunofluorescence Analysis: A representative lot detected a granular APC staining pattern at a subapical region of epithelial cells in fallopian mucosa by fluorescent immunohistochemistry staining of paraffin-embedded, paraformaldehyd-fixed fallopian tube tissue sections (Kessler, M., et al. (2012). Am. J. Pathol. 180(1):186-198). |
| Biological Information | |
|---|---|
| Immunogen | Human APC C-terminal fusion with MBP (maltose binding protein). |
| Epitope | C-terminal region. |
| Clone | C-APC 28.9 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Recognizes human APC, molecular weight is approximately 310 kDa. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | 311.6 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected APC in human cerebral cortex tissue. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |