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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IF, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB3802 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-53BP1 Antibody, clone BP13 |
| Alternate Names |
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| Background Information | P53-binding protein 1 (53BP1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, it is phosphorylated and relocalizes to the presumptive sites of damage, specifically, double-strand breaks. This also suggests a role in DNA repair, and maintaining genomic stability. It is sequence specific transcription factor that plays a central role in the the response of mammalian cells to genotoxic stress. Also, 53BP1 localizes to kinetochores in mitotic cells, suggesting a potential function in mitotic checkpoint signaling. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified immunoglobulin. Liquid in PBS, containing sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect 53BP1 also known as p53 Binding Protein 1 with Anti-53BP1 Antibody, clone BP13 (Mouse Monoclonal Antibody) validated in IF & WB. |
| Key Applications |
|
| Application Notes | Western blot. Recognizes a protein of >250 kD. Suggested dilution buffer is TBST. Suggested blocking buffer is 5% non-fat dry milk. Immunoprecipitation. Expected size in immunoblot is >250 kD. Suggested lysis buffer is NETN. Suggested capture agent is Protein A + rabbit anti-mouse linker antibody. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant human 53BP1 proteins. |
| Clone | BP13 |
| Host | Mouse |
| Specificity | 53BP1 |
| Isotype | IgG1 |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q12888 # May have a role in checkpoint signaling during mitosis (By similarity). Enhances TP53-mediated transcriptional activation. Binds to sites of DNA damage. Plays a role in the response to DNA damage. SIZE: 1972 amino acids; 213574 Da SUBUNIT: Interacts with IFI202A (By similarity). Binds to the central domain of TP53/p53. May form homo-oligomers. Interacts with DCLRE1C. Interacts with histone H2AFX and this requires phosphorylation of H2AFX on Ser-139. Interacts with histone H3 that has been methylated on Lys-79. Does not bind unmethylated histone H3. SUBCELLULAR LOCATION: Nucleus. Kinetochore. Note=Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. PTM: Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding. & Phosphorylated in an ATM-dependent manner in response to DNA damage. SIMILARITY: SwissProt: Q12888 ## Contains 2 BRCT domains. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |