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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, H, R | ELISA, IHC, IH(P), RIA, WB | M | Culture Supernatant | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB382 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Myelin Basic Protein Antibody, a.a. 129-138, clone 1 |
| Alternate Names |
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| Background Information | The classic group of MBP isoforms (isoforms 4-14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoforms 1-3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined to optional posttranslational modifications give a wide spectrum of isomers, each of them having maybe a specialized function. MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system. Isoform 1 Golli-MBP1, HOG7, 33 kDa Isoform 2 Golli-MBP2, HOG5, 21.5 kDa Isoform 3 MBP1, 21.5 kDa Isoform 4 MBP2, 20.2 kDa Isoform 5 MBP3, 18.5 kDa Isoform 6 MBP4, 17.2 kDa (SP_P02686) |
| Product Information | |
|---|---|
| Format | Culture Supernatant |
| Control |
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| Presentation | Culture supernatant containing 0.1 M Tris/HCl, pH 7.4 with 5-10% fetal calf serum and 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Myelin Basic Protein Antibody, a.a. 129-138, clone 1 is validated for use in ELISA, IH, IH(P), RIA, WB for the detection of Myelin Basic Protein. |
| Key Applications |
|
| Application Notes | Immunohistochemistry(paraffin) Analysis: Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Cerebellum Immunohistology on frozen sections at 1:10 Western Blot Analysis: A previous lot of this antibody was used in Western Blot. ELISA: A 1:200-1:1,000 dilution of a previous lot was used in ELISA. RIA: A previous lot of this antibody was used in Radioimmunoassay. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Bovine myelin basic protein |
| Epitope | a.a. 129-138 |
| Clone | 1 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Reacts with MBP from human, bovine and rat, epitope 129-138 |
| Isotype | IgG2a |
| Species Reactivity |
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| Species Reactivity Note | Reacts weakly with rabbit. Does not react with guinea pig. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. |
| Gene Symbol |
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| Purification Method | Unpurified |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P02686 # The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non- classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T- cells and neural cells. Differential splicing events combined to optional post-translational modifications give a wide spectrum of isomers, each of them having maybe a specialized function. Induces T-cell proliferation. SIZE: 304 amino acids; 33117 Da SUBUNIT: Homodimer; isoform 3 exists as a homodimer. SUBCELLULAR LOCATION: Myelin membrane; Peripheral membrane protein; Cytoplasmic side. Note=Cytoplasmic side of myelin. TISSUE SPECIFICITY: MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.DEVELOPMENTAL STAGE: Expression turns on abruptly in fetus of 14 to 16 weeks. Even smaller isoforms seem to be produced during embryogenesis, some of these persisting in the adult. Expression of isoform MBP2 is more evident at 16 weeks and its relative proportion declined thereafter. PTM: Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases in aging, making the protein more cationic. & The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6). & Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated. DISEASE: SwissProt: P02686 # The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease). SIMILARITY: SwissProt: P02686 ## Belongs to the myelin basic protein family. |
| Molecular Weight | 19 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by immunohistochemistry on brain tissue. Immunohistochemistry(paraffin) Analysis: MBP (cat. # MAB382) staining pattern/morphology in rat cerebellum. Tissue pretreated with Citrate, pH 6.0. This lot of antibody was diluted to 1:50, using IHC-Select® Detection with HRP-DAB. Immunoreactivity is seen as fiber staining in the junction between granular layer and molecular layer. Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Cerebellum |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20ºC in undiluted aliquots from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 2 mL |