Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | ELISA, ICC, WB | M | Ascites | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB3872 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-PPAR γ Antibody, isoform 1&2 |
| Alternate Names |
|
| Background Information | Peroxisome proliferator-activated receptors (PPARs) are nuclear receptors involved in lipid transport and metabolism. As such, their roles in chronic diseases such as diabetes, obesity, atherosclerosis and cancer are heavily investigated. Transcriptional activity of PPARs is regulated by fatty acid binding. Three PPAR isotypes have been identified: a, b and g. PPARg stimulates lipolysis of circulating triglycerides and the subsequent uptake of fatty acids into adipose cells. PPARs can bind to DNA only as a heterodimer with the retinoid X receptor (RXR). |
| Product Information | |
|---|---|
| Format | Ascites |
| Control |
|
| Presentation | Ascites fluid containing no preservatives. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-PPAR γ Antibody, isoform 1&2 is a Mouse Monoclonal Antibody for detection of PPAR gamma also known as Peroxisome Proliferator Activated Receptor γ & has been validated in ELISA, ICC & WB. |
| Key Applications |
|
| Application Notes | Western blot: 1:500-1:5,000 Immunocytochemistry: 1:500-1:5,000 ELISA: 1:500-1:5,000 Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide from amino acids 107-121 of human PPAR gamma2. |
| Epitope | isoform 1&2 |
| Clone | 1H4 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Reacts with human PPAR gamma2. The antibody also reacts with PPAR gamma1 due to immunogen sequence homology. The immunogen shows no homology with PPAR alpha or PPAR beta. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described. |
| Gene Symbol |
|
| Purification Method | Unpurified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P37231 # Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the receptor binds to a promoter element in the gene for acyl-CoA oxidase and activates its transcription. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. SIZE: 505 amino acids; 57620 Da SUBUNIT: Forms a heterodimer with the retinoic acid receptor RXRA called adipocyte-specific transcription factor ARF6. Interacts with NCOA6 coactivator, leading to a strong increase in transcription of target genes. Interacts with coactivator PPARBP, leading to a mild increase in transcription of target genes (By similarity). Interacts with NOCA7 in a ligand-inducible manner. Interacts with NCOA1 LXXLL motifs. Interacts with TGFB1I1. SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Highest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also are detectable in placenta, lung and ovary. DISEASE: SwissProt: P37231 # Defects in PPARG can lead to type 2 insulin-resistant diabetes and hyptertension. & Defects in PPARG may be associated with susceptibility to obesity [MIM:601665]. & Defects in PPARG may be associated with colon cancer. & Defects in PPARG are the cause of familial partial lipodystrophy type 3 (FPLD3) [MIM:604367]. Familial partial lipodystrophies (FPLD) are a heterogeneous group of genetic disorders characterized by marked loss of subcutaneous (sc) fat from the extremities. Affected individuals show an increased preponderance of insulin resistance, diabetes mellitus and dyslipidemia. & Variation in PPARG is associated with carotid intimal medial thickness 1 (CIMT1) [MIM:609338]. CIMT is a measure of atherosclerosis that is independently associated with traditional atherosclerotic cardiovascular disease risk factors and coronary atherosclerotic burden. 35 to 45% of the variability in multivariable-adjusted CIMT is explained by genetic factors. SIMILARITY: SwissProt: P37231 ## Belongs to the nuclear hormone receptor family. NR1 subfamily. & Contains 1 nuclear receptor DNA-binding domain. |
| Molecular Weight | 67 kDa |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |