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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Ma | IHC, IH(P), WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB388-100UG |
| Replaces | MAB388 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Myelin Proteolipid Protein Antibody, CT, clone PLPC1 |
| Alternate Names |
|
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse immunoglobulin in PBS, pH 7.2 containing 0.09% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Myelin Proteolipid Protein using this Anti-Myelin Proteolipid Protein Antibody, C-terminus, clone PLPC1 validated for use in IH, IH(P) & WB. |
| Key Applications |
|
| Application Notes | Western Blotting: detects a 23-25kDa protein and DM20 a 20kDa protein; PLP can aggregate causing HMW bands to sometimes appear in certain preparations. Immunocytochemistry: 4% PFA fixed cells, 5-10 minutes room temperature. Use 0.1% triton in block only to allow antibody access to C-terminal epitope. Immunohistology on fresh frozen sections or 4% PFA fixed, frozen tissues. Epitope is internal, use 0.1-0.2% triton X-100 in the blocking step for permeabilization. Immunohistochemistry in paraffin: works best with lightly fixed tissues, or Bouins fixed material, although there are reports of traditional formalin fixation also working with antigen recovery. {Mathis, et al 2003}. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Myelin proteolipid protein, synthetic peptide CGRGTKF corresponding to the C-terminal peptide. |
| Epitope | C-terminus |
| Clone | PLPC1 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Reacts with myelin proteolipid protein in many mammalian species. Shows cross-reactivity to DM20. |
| Isotype | IgG2a |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a transmembrane proteolipid protein that is the predominant myelin protein present in the central nervous system. It may play a role in the compaction, stabilization, and maintenance of myelin sheaths, as well as in oligodendrocyte development and axonal survival. Mutations in this gene cause X-linked Pelizaeus-Merzbacher disease and spastic paraplegia type 2. Alternatively spliced transcript variants encoding distinct isoforms or having different 5 UTRs, have been identified for this gene. |
| Gene Symbol |
|
| Purification Method | Protein A Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P60201 # This is the major myelin protein from the central nervous system. It plays an important role in the formation or maintenance of the multilamellar structure of myelin. SIZE: 277 amino acids; 30,077 Da SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. PTM: Lipoprotein, Palmitate DISEASE:Defects in PLP1 are the cause of Pelizaeus-Merzbacher disease (PMD) [MIM:312080]. PMD is an X-linked neurologic disorder of myelin metabolism. It is characterized by early impairment of motor development (during the first three months of life) and, later, by the development of abnormal movements and progressive spastic paraplegia. SIMILARITY: Belongs to the myelin proteolipid protein family. |
| Molecular Weight | 23-25 kDa |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |