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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | ICC, IH(P), FUNC | M | Saporin | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB390-25UG |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-p75 LNGFR Antibody, Saporin conjugated, clone 192 |
| Alternate Names |
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| Background Information | The Low Affinity Nerve Growth Factor Receptor (also called the LNGFR or p75 neurotrophin receptor) is one of the two receptor types for the neurotrophins, a family of protein growth factors which stimulate neuronal cells to survive and differentiate. The neurotrophins are comprised of four proteins, all of which bind to the LNGFR: Nerve Growth Factor (NGF), Brain Derived Neurotrophic Factor (BDNF), Neurotrophin-3 (NT-3), and Neurotrophin-4 (NT-4. Nerve growth factor, the prototypical growth factor, is a protein secreted by a neurons target. NGF is critical for the survival and maintenance of sympathetic and sensory neurons. NGF is released from the target cells, binds to and activates its high affinity receptor (TrkA), and is internalized into the responsive neuron. The NGF/TrkA complex is subsequently trafficked back to the cell body. This movement of NGF from axon tip to soma is thought to be involved in the long-distance signaling of neurons. |
| Product Information | |
|---|---|
| Format | Saporin |
| Control |
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| Presentation | Saporin Conjugated. 192-IgG-SAP is sterile filtered and presented in Dulbeccos PBS with no preservative. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-p75 Low Affinity Nerve Growth Factor Receptor Antibody, Saporin conjugated, clone 192 is validated for use in ICC, IHC(P), FUNC for the detection of p75 Low Affinity Nerve Growth Factor Receptor. |
| Key Applications |
|
| Application Notes | Immunohistochemistry (Paraffin): A previous lot of this antibody was used in IH(P). Immunocytochemistry: A previous lot of this antibody was used in IC. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | p75-expressing cells |
| Clone | 192 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Recognizes the rat NGF receptor (p75, low affinity Neurotrophin Receptor). MAB365 is the monoclonal antibody used for MAB390-SAP. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. |
| Gene Symbol |
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| Non-Reactive Species |
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| Purification Method | Protein A Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P08138 # Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. SIZE: 427 amino acids; 45183 Da SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR- associated cell death executor. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1. Interacts with BEX1 and NGFRAP1/BEX3. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. DOMAIN: SwissProt: P08138 Death domain is responsible for interaction with RANBP9. PTM: N- and O-glycosylated. & O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc. & Phosphorylated on serine residues. SIMILARITY: Contains 1 death domain. & Contains 4 TNFR-Cys repeats. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Centrifuge material at low speed in microfuge to ensure all of the solution is at the bottom of the tube. Vortex gently. Long term storage at -70ºC or below. Short term storage at -20ºC or 2-8ºC with appropriate antibacterial agent if compatible with the intended application. The sterile conjugate is stable at 2-8ºC for several months. Avoid repeated freezing and thawing. Glycerol (1:1) can be added for additional stability if compatible with the intended application. This immunotoxin may be handled safely using standard laboratory procedures as the cross-species reactivity between these anti-rat p75 antibodies and the human form of p75 is negligible. |
| Packaging Information | |
|---|---|
| Material Size | 25 µg |