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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC, IHC, IH(P), IP, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB4092 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-P-Selectin Glycoprotein Ligand-1 Antibody, clone KPL-1 |
| Alternate Names |
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| Background Information | Interactions between P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) mediate the earliest “rolling” of leukocytes on the lumenal surface of endothelial cells at sites of inflammation. To date, the primary role of PSGL-1 in mediating the interaction between neutrophils and P-selectin has been well documented. MAB4092 completely blocks recognition of PSGL-1 by either P-selectin or by L-selectin, but does not affect leukocyte recognition of E-selectin. Two color flow cytometry of normal leukocytes has demonstrated that PSGL-1 is expressed on essentially all blood neutrophils, NK cells, blood cells, T cells, and monocytes, but PSGL-1 stains B cells at significantly lower levels than other cell types. Variation in tyrosine sulfation during B cell differentiation may affect the stability of B cells to interact with P- and L-selectin. {Snapp, KR et al 1998}. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified Immunoglobulin. Liquid in 0.02M PB with 0.25M NaCl, pH7.6, 0.1% Sodium Azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect P-Selectin Glycoprotein Ligand-1 using this Anti-P-Selectin Glycoprotein Ligand-1 Antibody, clone KPL-1 validated for use in FC, IH, IH(P), IP & WB. |
| Key Applications |
|
| Application Notes | Western blot of HL60 whole cell lysates prepared with 1% triton X-100 in 150mM NaCl, 10mM Tris-HCL pH 7.6, 1 mM Ca++, 1 mM Mg++, with 1mmol aprotinin, PMSF,leupeptin, and pepstatin A {Snapp, KR et al 1998}. 1:1000 FACS {Snapp, KR et al 1998}. Immunoprecipitation: 0.5% triton X-100 in TBS with proteinase inhibitors; 5 µg/500µL of whole cell lysate (350-500mg/mL total protein). Functional blocking of P-selectin {Snapp KR et al 1998}. Immunohistochemistry: Fresh frozen, acetone fixed sections Immunohistochemistry in paraffin embedded tissues: formalin fixed, pretreatment standard citric acid pH 6.0, microwave treatment is recommended 1:50-1:200 Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | The antibody epitope was mapped to a site within a consensus tyrosine sulfation motif of PSGL-1, previously shown to be essential for interaction with P-selectin (and now shown to be essential for recognition of PSGL-1 by L-selectin). |
| Clone | KPL-1 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | P-selectin glycoprotein ligand-1 (PSGL-1). The antibody epitope was mapped to a site within a consensus tyrosine sulfation motif of PSGL-1, previously shown to be essential for interaction with P-selectin (and now shown to be essential for recognition of PSGL-1 by L-selectin). {Snapp KR et al. 1998}. The binding of KPL-1 to human PSGL-1 on HL-60 cells does not require sulfation {Thatte, A et al 2002}. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Known not to react with Rat, Bovine, Pig, and Equine PSGL-1 {Snapp, KR et al 1998; Baisse, B et al 2007}. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | SELPLG is the high affinity counter-receptor for P-selectin on myeloid cells and stimulated T lymphocytes. As such, it plays a critical role in the tethering of these cells to activated platelets or endothelia expressing P-selectin. The organization of the SELPG gene closely resembles that of CD43 and the human platelet glycoprotein GpIb-alpha both of which have an intron in the 5-prime-noncoding region, a long second exon containing the complete coding region, and TATA-less promoters. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q14242 # Binds to P-, E- and L-selectins. The calcium-dependent high affinity interaction with P-selectin mediates the tethering and rolling of neutrophils and T-lymphocytes on endothelial cells. SIZE: 412 amino acids; 43201 Da SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Expressed on neutrophils, monocytes and most lymphocytes. PTM: Displays complex, sialylated and fucosylated O-linked oligosaccharides, at least some of which appear to contain poly-N- acetyllactosamine with varying degrees of substitution. Also N- glycosylated. & Sulfated in the N-terminal region; sulfation is necessary for P-selectin binding. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at 2-8°C in undiluted aliquots up to 12 months. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |