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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC, WB, ICC, IHC, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB4100 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-MRP1 Antibody, clone QCRL-1 |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified immunoglobulin. Liquid in PBS with 0.1% sodium azide. |
| Quality Level | MQ300 |
| Applications | |
|---|---|
| Application | Anti-MRP1 Antibody, clone QCRL-1 detects level of MRP1 & has been published & validated for use in FC, WB, IC, IH, IH(P). |
| Key Applications |
|
| Application Notes | Western Blot: 0.2 μg/mL, with HRP-conjugated anti mouse IgG (Hipfner et al., 1994, 1996). Immunocytochemistry: 1:20 - 1:50 on paraformaldehyde-fixed (0.5% in PBS) or 70% methanol-fixed cytospin preparations (Hipfner et al., 1999). Immunohistochemistry on formalin-fixed paraffini-embedded sections following antigen retrieval by microwave heating in citrate (Wright, et al., 1998). Flow cytometry: 1:20 dilution on cells fixed in 0.5% paraformaldehyde in PBS for 30 minutes, followed by anti-mouse FITC (block and wash with solution containing 0.1% Tween) (Hipfner, et al., 1994). Immunoprecipitation (Hipfner, et al., 1994; 1996). Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Non-denatured membranes prepared from the human small cell lung cancer cell line, H69AR, which highly overexpresses MRP1 (Cole et al., 1992; Hipfner et al., 1994, 1999). |
| Clone | QCRL-1 |
| Host | Mouse |
| Specificity | Reacts with a defined linear epitope (aa 918-924) of MRP1, a 190 kDa transmembrane phosphoglycoprotein overexpressed in various human multidrug resistant tumor cell lines and tumors (Cole et al., 1992; Hipfner et al., 1994, 1996, 1999; Wright et al, 1998). Does not cross react with the mouse mrp1, or human MDR1, MDR3, MRP2, MRP3, MRP4, MRP5 or MRP6 gene products. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra-and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This full transporter is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions as a multispecific organic anion transporter, with oxidized glutatione, cysteinyl leukotrienes, and activated aflatoxin B1 as substrates. This protein also transports glucuronides and sulfate conjugates of steroid hormones and bile salts. Alternative splicing by exon deletion results in several splice variants but maintains the original open reading frame in all forms. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P33527 # May participate directly in the active transport of drugs into subcellular organelles or influence drug distribution indirectly. Confers resistance to anticancer drugs. Transports LTC4. May protect milk against xenobiotics (By similarity). SIZE: 1531 amino acids; 171561 Da SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Lung, testis and peripheral blood mononuclear cells. SIMILARITY: SwissProt: P33527 ## Belongs to the ABC transporter family. Conjugate transporter (TC 3.A.1.208) subfamily. & Contains 2 ABC transmembrane type-1 domains. & Contains 2 ABC transporter domains. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Store at 2-8ºC for up to 3 months. For longer term storage, aliquot and freeze at -20ºC. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |