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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | ELISA, FC, IF, IHC, IH(P), IP, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB4130 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Thymidylate Synthase Antibody, clone TS106 |
| Alternate Names |
|
| Background Information | TS (EC:2.1.1.45), a cytosolic enzyme, is a dimmer of two identical monomers of about 36kDa. The enzyme provides the sole intracellular de novo source of thymidylate and plays a crucial role in DNA replication and repair. TS catalyzes the methylation of deoxyuridine monophosphate (dump) and its conversion to deoxythymidine monophosphate (dTMP). Therefore, TS is primarily active in proliferating and metabolic active cells. TS is a central target of the widely used antineoplastic agent 5-Fluorouracil (5-FU) and thus also of the Xeloda, which is enzymatically activated to 5-FU. TS is inactivated by a covalent complex formation with 5-FdUMP and methylenetetrahydrofolate. Literature indicates that expression of TS is associated with response to 5-fluorouracil (5-FU) in human breast, colorectal, gastric, head, and neck carcinomas with low TS expression predicting better response to 5-FU and survival. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG1 in 0.02M PBS with 0.25M NaCl, pH=7.6, 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Use Anti-Thymidylate Synthase Antibody, clone TS106 (Mouse Monoclonal Antibody) validated in ELISA, FC, IF, IHC, IHC(P), IP, WB to detect Thymidylate Synthase also known as Thymidylate synthase. |
| Key Applications |
|
| Application Notes | ELISA: A previous lot of this antibody was used in ELISA. Flow Cytometry: A previous lot of this antibody was used in FC. Immunofluorescence: A previous lot of this antibody was used in IF. Immunoprecipitation: A 10 μL/mg concentration of a previous lot was used on protein lysate. Immunohistochemistry (Frozen and Paraffin sections): 1:50 - 1:100 (with incubation for 30-60 minutes at room temperature). TS106 can benefit from antigen retrieval with either EDTA pH 8.0 or 10mM citrate buffer pH 6.0 . Western Blotting: 1:200 - 1:400. Recognizes a 36KDa band. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant Thymidylate Synthase. |
| Clone | TS106 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Antibody MAB4130 recognizes a 36 kDa protein identified as Thymidylate Synthase [TS], which is a target for the fluoropyrimidine group of antineoplastic drugs used to treat solid tumors. Expression of TS is associated with response to 5-fluorouracil (5-FU) in human breast, colorectal, gastric, head, and neck carcinomas. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Species Reactivity Note | Human and mouse. Expected to react with rat based on sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Thymidylate synthase (TYMS, or TS; EC 2.1.1.45) uses the 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor to maintain the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs.[supplied by OMIM] |
| Gene Symbol |
|
| Purification Method | Protein A Purfied |
| UniProt Number |
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| UniProt Summary | SIZE: 313 amino acids; 35716 Da SUBUNIT: Homodimer. SIMILARITY: SwissProt: P04818 ## Belongs to the thymidylate synthase family. |
| Molecular Weight | 36 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot on MCF7 lysates. Western Blot Analysis: 1:500 dilution of this antibody detected THYMIDYLATE SYNTHASE on 10 μg of MCF7 lysates. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8ºC from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |