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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, ICC, IHC | M | Culture Supernatant | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB4140 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-MDR3 Antibody, clone P3 II-26 |
| Product Information | |
|---|---|
| Format | Culture Supernatant |
| Presentation | Liquid containing 0.7% BSA and 0.1% sodium azide. |
| Quality Level | MQ300 |
| Applications | |
|---|---|
| Application | Anti-MDR3 Antibody, clone P3 II-26 is an antibody against MDR3 for use in WB, IC, IH. |
| Key Applications |
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| Applications Not Recommended |
|
| Application Notes | Western Blotting: MDR3 is a 140 kDa protein. Immunocytochemistry: 1:20 - 1:50 on acetone-fixed cytospin preparations Immunohistochemistry: 1:20 on acetone-fixed frozen sections Not suitable for paraffin-embedded tissues Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Mab was selected after immunization with a fusion protein consisting of Gluthatione-S-Transferase and a fragment of MDR3 P-gp comprising aa 629-692. |
| Clone | P3 II-26 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody reacts with an internal epitope of MDR3 P-gp. It does not cross-react with the human MDR1 P-gp. It has been reported that this clone also cross reacts with mouse MDR2 Pgp {Kok et al 2003 {http://www.biochemj.org/bj/369/0539/3690539.pdf}. |
| Isotype | IgG2b |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. This gene encodes a full transporter and member of the p-glycoprotein family of membrane proteins with phosphatidylcholine as its substrate. The function of this protein has not yet been determined; however, it may involve transport of phospholipids from liver hepatocytes into bile. Alternative splicing of this gene results in several products of undetermined function. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P21439 # Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells. Human MDR3 is not capable of conferring drug resistance. Mediates the translocation of phosphatidylcholine across the canalicular membrane of the hepatocyte. SIZE: 1286 amino acids; 141523 Da SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. DISEASE: SwissProt: P21439 # Defects in ABCB4 are the cause of progressive familial intrahepatic cholestasis type III (PFIC3) [MIM:602347]. PFIC3 is an autosomal recessive liver disorder presenting with early onset cholestasis that progresses to cirrhosis and liver failure before adulthood. It is characterized by elevated serum gamma- glutamyltransferase levels. & Defects in ABCB4 are the cause of intrahepatic cholestasis of pregnancy (ICP) [MIM:147480]; also known as obstetric cholestasis. ICP is a liver disorder of pregnancy with serious consequences for the mother and fetus. Cholestasis results from abnormal biliary transport from the liver into the small intestine, and ICP is characterized by the occurrence of cholestasis in pregnancy in women with an otherwise normal medical history. The classical maternal feature is generalized pruritus, becoming more severe with advancing gestation. ICP causes fetal distress, spontaneous premature delivery and unexplained third trimester intrauterine death. & Defects in ABCB4 are a cause of cholelithiasis [MIM:600803]; also known as gallstones. SIMILARITY: SwissProt: P21439 ## Belongs to the ABC transporter family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. & Contains 2 ABC transmembrane type-1 domains. & Contains 2 ABC transporter domains. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |