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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, ICC, IHC | M | Culture Supernatant | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB4148 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-MRP2 Antibody, clone M2 I-4 |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Culture Supernatant |
| Presentation | Liquid containing 0.7% BSA and 0.1% sodium azide. |
| Quality Level | MQ300 |
| Applications | |
|---|---|
| Application | This Anti-MRP2 Antibody, clone M2 I-4 is validated for use in WB, IC, IH for the detection of MRP2. |
| Key Applications |
|
| Application Notes | Western blotting: 1:20 - 1:50 with anti-mouse HRP Immunocytochemistry: 1:20 - 1:50 on acetone fixed cytospin preparations Immunohistochemistry: 1:20 on acetone fixed frozen tissue sections Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Fusion protein of MRP2 containing aa 215-310 of the protein. |
| Clone | M2 I-4 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody reacts with an internal epitope of MRP2, a 190-200 kDa transmembrane protein previously known as the canalicular multi-organic anion transporter (cMOAT). MRP2 is a member of the MRP family of multidrug resistance related proteins, and MRP2 overexpression has been observed in a subset of cisplatin resistant cell lines. This antibody does not cross-react with the human MDR1, MRP1, MRP3 or MRP5 gene products. |
| Isotype | IgG1 |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein is expressed in the canalicular (apical) part of the hepatocyte and functions in biliary transport. Substrates include anticancer drugs such as vinblastine; therefore, this protein appears to contribute to drug resistance in mammalian cells. Several different mutations in this gene have been observed in patients with Dubin-Johnson syndrome (DJS), an autosomal recessive disorder characterized by conjugated hyperbilirubinemia. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q92887 # Mediates hepatobiliary excretion of numerous organic anions. May function as a cellular cisplatin transporter. SIZE: 1545 amino acids; 174191 Da SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Found on the apical membrane of polarized cells in liver, kidney and intestine. The highest expression is found in liver. DISEASE: SwissProt: Q92887 # Defects in ABCC2 are the cause of Dubin-Johnson syndrome (DJS) [MIM:237500]. DJS is an autosomal recessive disorder characterized by conjugated hyperbilirubinemia, an increase in the urinary excretion of coproporphyrin isomer I, deposition of melanin-like pigment in hepatocytes, and prolonged retention of sulfobromophthalein, but otherwise normal liver function. SIMILARITY: SwissProt: Q92887 ## Belongs to the ABC transporter family. Conjugate transporter (TC 3.A.1.208) subfamily. & Contains 2 ABC transmembrane type-1 domains. & Contains 2 ABC transporter domains. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain refrigerated at 2-8°C for up to 6 months. For long-term storage store at -20°C. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |