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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC, IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB4162 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-MDR1 Antibody, extracellular human specific Pgp, clone MM4.17 |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative. |
| Quality Level | MQ300 |
| Applications | |
|---|---|
| Application | Anti-MDR1 Antibody, extracellular human specific Pgp, clone MM4.17 is an antibody against MDR1 for use in FC, IH. |
| Key Applications |
|
| Application Notes | Flow Cytometry Immunohistochemistry on formalin fixed paraffin embedded sections Confocal and Electron Microscopy ELISA Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Living intact human multidrug-resistant cells |
| Epitope | extracellular human specific Pgp |
| Clone | MM4.17 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Recognizes a mapped epitope in the extracellular Pgp domain and detects low-level Pgp expression in living (intact) human MDR cells (Cianfriglia, 2002). The epitope has been mapped to the single amino acid level using different techniques (Cianfriglia, 1994 and Poloni, 1995). |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. |
| Gene Symbol |
|
| Purification Method | Protein A Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P08183 # Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells. SIZE: 1280 amino acids; 141463 Da SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Expressed in liver, kidney, small intestine and brain. SIMILARITY: SwissProt: P08183 ## Belongs to the ABC transporter family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. & Contains 2 ABC transmembrane type-1 domains. & Contains 2 ABC transporter domains. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |