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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC, WB, ICC, IHC, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB4197 |
| Replaces | MABE519 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Topoisomerase II Antibody, clone KiS1 |
| Alternate Names |
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| Background Information | Topoisomerase II β (M.W 180 kDa) plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is present ubiquitously in normal cells and is upregulated in tumors and proliferating cells. Topoisomerase II β is also implicated in drug resistance of tumor cells. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified immunoglobulin. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Topoisomerase II Antibody, clone KiS1 is a Mouse Monoclonal Antibody for detection of Topoisomerase II also known as Ki-S1 & has been validated in FC, WB, ICC, IHC, IHC(P). |
| Key Applications |
|
| Application Notes | Western blot: 1-10μg/ml Immunoprecipitaion Immunocytochemistry: 5-10 μg/ml Immunohistochemistry: 5-10 μg/ml Flow cytometry Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Nuclear protein preparation from the human cell line U937.50 |
| Clone | KiS1 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | In Immunoprecipitation and Western blot experiments the Ki-S1 antibody recognizes a major protein of 170 kD which was identified as the a isoform of topoisomerase II (Boege et al., 1995; Kreipe et al., 1993). In addition, it has been shown that the Ki-S1 antibody recognizes a carboxyterminal a-isoenzyme specific epitope missing in topoisomerase IIb (Boege et al., 1995). In immunohistochemistry the Ki-S1 antibody shows strong nuclear staining only in proliferating cells. The epitope recognized by the antibody is also detectable in paraffin-embedded tissue sections (Kreipe et al., 1993). Accordingly, it has been shown that the expression of topoisomerase IIa is strongly restricted to proliferating cells (Tsutsui et al., 1993). The topoisomerase IIa antigen is preferentially expressed during G 1 , S, G 2 and M phase of the cell cycle, while resting, non-cycling cells (G 0 phase) lack topoisomerase IIa. In addition, constantly proliferating cells (e.g. cell lines) react positively to topoisomerase IIa during the entire cell-cycle. This specificity of Ki-S1 antibody for proliferating cells might make it a useful tool for determination of the proliferative fraction in solid tumors such as mammary carcinomas (Kreipe et al., 1993; Sampson et al., 1992; Camplejohn et al., 1993; Kreipe et al., 1993; Rasbridge et al., 1994) and gangliomas (Wolf et al., 1994). |
| Isotype | IgG2a |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P11388 # Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. SIZE: 1531 amino acids; 174385 Da SUBUNIT: Homodimer. Interacts with COPS5. SUBCELLULAR LOCATION: Cytoplasm. Nucleus, nucleoplasm. Note=Generally located in the nucleoplasm. PTM: Phosphorylation has no effect on catalytic activity. SIMILARITY: SwissProt: P11388 ## Belongs to the type II topoisomerase family. MISCELLANEOUS: Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at 2-8°C in undiluted aliquots for up to 6 months.Avoid repeat freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |