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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | FC, ICC, IF, WB | M | FITC | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB4212F |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-HU-B27 Antibody, FITC conjugated |
| Alternate Names |
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| Background Information | HU-B27 (HLA- Com-B27) is involved in the presentation of foreign antigens to the immune system. HLA B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta 2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. |
| Product Information | |
|---|---|
| Format | FITC |
| Control |
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| Presentation | The reagent is supplied in 1.0 mL phosphate-buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide. Purified from mouse ascitic fluid. The characteristics of each lot are tested by gel electrophoresis and flow cytometry. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect HU-B27d using this Anti-HU-B27 Antibody, FITC conjugated validated for use in FC, IC, IF & WB. |
| Key Applications |
|
| Application Notes | Flow cytometry and Immunofluorescence: use 10 mL direct from the vial per 100 mL of whole blood, or 1 x 106 peripheral blood mononuclear cells (PBMC) in 100 mL buffer. Flow cytometric analysis of lymphocyte staining for HLA-B27 gave the following results: Subjects/Fluorescence Intensity Heterozygous HLA-B27+/Strong Homozygous HLA-B7+/Weak Heterozygous HLA-B7+/Weak - negative Non B27, Non B7/Negative This reagent is useful in the investigation of HLA-B27 in diseases such as ankylosing spondylitis. SUGGESTED USAGE: Flow cytometry and immunofluorescence - use 10 µl direct from the vial per 100 µl of whole blood, or 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 µl buffer. Use Control IgG2a-FITC (MABC002F) as an isotype control. Please note that this reagent should be used in a single-colour single-step assay only. |
| Biological Information | |
|---|---|
| Host | Mouse |
| Specificity | This reagent binds strongly to the HLA-B27 antigen. It also binds weakly to the HLAB7 antigen. This formulation demonstrates less cross reactivity to HLA-B7 than Chemicon products, MAB1285 and MAB1285F. |
| Isotype | IgG2a |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
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| UniProt Number |
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| Molecular Weight | 19 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot on Jurkat lysates. Western Blotting Analysis: 1:500 dilution of this antibody detected Com-B27 on 10 µg of Jurkat lysates. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 6 months at 2-8ºC in the dark from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 1 mL |