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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | FC, ICC, IF | M | AlexaFluor®488 | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB4301X |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Stage-Specific Embryonic Antigen-1 Antibody, clone MC-480, Alexa Fluor® 488 |
| Alternate Names |
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| Background Information | SSEA-1 is expressed on the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Expression of SSEA-1 is down regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is characterized by an increase in SSEA-1 expression. |
| Product Information | |
|---|---|
| Format | AlexaFluor®488 |
| Control |
|
| Presentation | Purified immunoglobulin conjugated to Alexa Fluor® 488. Liquid in Phosphate buffer with 15 mg/mL BSA as a stabilizer and 0.1% sodium azide |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Stage-Specific Embryonic Antigen-1 Antibody, clone MC-480, Alexa Fluor 488 is validated for use in FC, IC, IF for the detection of Stage-Specific Embryonic Antigen-1. |
| Key Applications |
|
| Application Notes | FACS Immunocytochemistry: 1. Fix cells with a 4% Paraformaldehyde solution for 30-45 minutes, and then rinse several times with Tris-Buffered-Saline (TBS). 2. Incubate cells with MAB4301X diluted to 1/100 with PBS/ Tween-20 (0.05%) / BSA (1%) for 30 minutes. 3. Wash the sample three times with PBS / Tween-20 (0.05%) for 1 minute per wash. Examine under a microscope fitted with a FITC filter. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | F9 teratocarcinoma stem cells (X-irradiated) |
| Clone | MC-480 |
| Host | Mouse |
| Specificity | Reacts with the Stage-specific embryonic antigen-1 (SSEA-1) that is expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Expression of SSEA-1 is down regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is characterized by an increase in SSEA-1 expression. |
| Isotype | IgM |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | FUT4 catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15). It has been detected in human embryos (5-10 weeks) suggesting a role in development. |
| Gene Symbol |
|
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P22083 # May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens. SIZE: 405 amino acids; 45570 Da SUBCELLULAR LOCATION: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Membrane-bound form in trans cisternae of Golgi. SIMILARITY: SwissProt: P22083 ## Belongs to the glycosyltransferase 10 family. |
| Stem Cell Type |
|
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain refrigerated at 2–8°C protected from light in undiluted aliquots for up to 24 months. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |