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Millipore Anti-Stage-Specific Embryonic Antigen-4 Antibody, Clone Mc-813-70 Is An Antibody Against Stage-Specific Embryonic Antigen-4 For Use In Fc, Elisa, If, Ih. - Mill (Additional S&H or Hazmat Fees May Apply)
The cell-surface markers used routinely to identify undifferentiated human embryonic stem cells (hESCs) were initially characterized as markers for mESCs, mouse embryonic carcinomas (ECs), or human EC cells. Stage-specific embryonic antigen-3 (SSEA-3) and SSEA-4 are widely used as cell-surface markers to define both human and mouse ESCs. SSEA-3 and SSEA-4 are expressed on undifferentiated hESCs but not on undifferentiated mESCs.
Product Information
Format
Purified
Control
Pluripotent human embryonic stem (ES) cells
Presentation
Purified moust monoclonal IgG3 liquid in buffer containing 0.02 M PBS, 0.25 M NaCl, pH 7.6 with 0.1% Sodium Azide
Quality Level
MQ100
Applications
Application
Anti-Stage-Specific Embryonic Antigen-4 Antibody, clone MC-813-70 is an antibody against Stage-Specific Embryonic Antigen-4 for use in FC, ELISA, IF, IH.
Key Applications
Flow Cytometry
ELISA
Immunofluorescence
Immunohistochemistry
Application Notes
Immunohistochemistry: A previous lot of this antibody was used in IH.
Immunofluorescence: A previous lot of this antibody was used in IF.
ELISA: A previous lot of this antibody was used in ELISA.
Flow Cytometry: A starting range of 10-20 µg/mL is suggested.
Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen
Human embryonal carcinoma cell line 2102Ep
Clone
clone MC-813-70
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
This antibody reacts with the Stage-specific embryonic antigen-4 (SSEA-4) that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is evident with undifferentiated murine EC, ES and EG cells. Expression of SSEA-4 is down regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression.
Isotype
IgG3
Species Reactivity
Human
Mouse
Antibody Type
Monoclonal Antibody
Gene Symbol
SSEA-4
SSEA4
Purification Method
Protein A Purfied
Stem Cell Type
Human Embryonic Stem Cells
Mouse Embryonic Stem Cells
Induced Pluripotent Stem Cells
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
