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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | ELISA, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB4307 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Mouse Leukemia Inhibitory Factor Antibody, clone 2H2.2 |
| Alternate Names |
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| Background Information | Leukemia inhibitory factor, or LIF, is a pleiotropic cytokine that is expressed by a wide variety of cells including activated T lymphocytes, monocytes, mast cells and neuronal cells. Human LIF cDNA encodes a 202 amino acid precursor which is processed to yield a 180 amino acid, biologically active glycoprotein with an approximate molecular weight of 38-67 kDa. In addition to its involvement in the stimulation of acute-phase protein during inflammatory processes, LIF has also been implicated in promoting the survival and growth of axons in vitro. LIF signals through the LIF receptor, a heterodimer comprised of a 190 kDa LIFR subunit and the gp130 subunit common to the IL-6, IL-11, CNTF and OSM receptors. Janus tyrosine kinases, JAK1 and JAK2, are activated in response to LIF stimulation, which in turn phosphorylate Stat1 and Stat3 causing their nuclear translocation and the transcription of Stat-responsive genes. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified Immunoglobulin. Liquid in 0.02M PBS, 0.25M NaCl pH 7.6 with 0.1% Sodium Azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Mouse Leukemia Inhibitory Factor Antibody, clone 2H2.2 is an antibody against Mouse Leukemia Inhibitory Factor for use in ELISA & WB. |
| Key Applications |
|
| Application Notes | Immunoblotting: a starting dilution of 1-10 μg/mL is suggested. EIA Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant Murine LIF. |
| Clone | 2H2.2 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody is reactive to E.coli and STO derived recombinant murine Leukemia Inhibitory Factor (LIF). |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Summary | Leukaemia inhibitory factor is a cytokine that induces macrophage differentiation. Neurotransmitters and neuropeptides, well known for their role in the communication between neurons, are also capable of activating monocytes and macrophages and inducing chemotaxis in immune cells. LIF signals through different receptors and transcription factors. LIF in conjunction with BMP2 acts in synergy on primary fetal neural progenitor cells to induce astrocytes. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P15018 # LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. SIZE: 202 amino acids; 22008 Da SUBCELLULAR LOCATION: Secreted. SIMILARITY: SwissProt: P15018 ## Belongs to the LIF/OSM family. |
| Stem Cell Type |
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| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |