Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB4356 |
| Description | Anti-DPPA-2 Antibody, clone 6C1.2 |
| Alternate Names |
|
| Background Information | DPPA-2 (developmental pluripotency associated-2) is a novel Oct-4-related protein that is localized to the nucleus of mouse fetal germ cells. DPPA-2 has binding sites for both POU and Sox-2 protein domains and is expressed primarily in the developing germ line following gastrulation. These associations suggest that this protein participates in a very strong supportive role in maintaining cell pluripotentiality. In addition to its role as a pluripotency marker, more recent research using cancer cells suggests that DPPA-2 may also act as an embryo-cancer antigen—pointing to the presence of abnormal cell growth and the formation of cancerous cells. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4) and 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-DPPA-2 Antibody, clone 6C1.2 is validated for use in WB for the detection of DPPA-2. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | Full length recombinant mouse DPPA-2 protein. |
| Clone | 6C1.2 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This monoclonal antibody recognizes the mouse DPPA-2 protein. |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Species Reactivity Note | Reacts with mouse. Reactivity with other species has not been tested. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein g Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: May play a role in maintaining cell pluripotentiality. SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Expressed in embryonic stem cells. No expression is seen in 5 months embryo, mesenchymal stem cells, embryonic fibrocytes and adult tissues. SEQUENCE SIMILARITIES: Contains 1 SAP domain. |
| Stem Cell Type |
|
| Molecular Weight | ~34 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | (Based upon representative data from a previous lot). Western Blotting: Recommended working concentration is 2 µg/mL. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |