The store will not work correctly when cookies are disabled.
We use cookies to make your experience better.To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
NETA ALERT: Due to inclement weather our offices are closed today, Monday Feb. 23. We will not be shipping orders.
Millipore Detect Srf [Serum Response Factor] Using This Anti-Srf [Serum Response Factor] Antibody, Clone 1e1 Validated For Use In Wb & Ic. - Mill (Additional S&H or Hazmat Fees May Apply)
Serum response factor (SRF) is a nuclear transcription factor that binds to the serum response element (SRE), and is essential for embryonic development of the mesoderm, as well as for the maintenance of adult muscle cells and neurons. As this protein is involved in the downstream regulation of many immediate-early genes, it participates in a variety of cell functions, including cell cycle, apoptosis, cell growth, and cell differentiation. SRF is most commonly activated by serum, LPA, LPS, TPA, growth factors, including TNFalpha, as well as antioxidants and UV light.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, and 0.05% sodium azide.
Applications
Application
Detect SRF [Serum Response Factor] using this Anti-SRF [Serum Response Factor] Antibody, clone 1E1 validated for use in WB & IC.
Key Applications
Western Blotting
Immunocytochemistry
Application Notes
Immunocytochemistry: Recommended working dilution is 1:500.
Biological Information
Immunogen
Synthetic human SRF peptide, a.a. 406-508.
Clone
1E1
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
This monoclonal antibody recognizes SRF.
Isotype
IgG1κ
Species Reactivity
Human
Mouse
Species Reactivity Note
Reacts with human. A weak interaction with mouse has also been observed. Reactivity with other species has not been tested.
Antibody Type
Monoclonal Antibody
Entrez Gene Number
P11831
Gene Symbol
ELK3
ERP
MCM1
OTTHUMP00000039820
SAP2
Purification Method
Protein G Purified
UniProt Number
P11831
UniProt Summary
FUNCTION: SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5 of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentation and maturation.
SUBUNIT STRUCTURE: Binds DNA as a multimer, probably a dimer. Interacts with MLLT7/FOXO4, NKX3A and SSRP1. Interacts with ARID2 and SRFBP1 By similarity. Forms complexes with ARID2, MYOCD, NKX2-5 and SRFBP1 By similarity.
(Based upon representative data from a previous lot).
Western Blotting: Recommended working dilution is 0.5 µg/mL.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months. Avoid repeat freeze/thaw cycles.
