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Millipore Anti-Tra-1-81 Antibody, Clone Tra-1-81 Detects Level Of Tra-1-81 & Has Been Published & Validated For Use In Wb, Fc, If, Ip & Ic. - Mill (Additional S&H or Hazmat Fees May Apply)
Human embryonal carcinoma (EC) cells are the stem cells of teratocarcinomas, and they are key components of germ cell tumors (GCTs). Embryonal carcinoma, as do all human pluripotent stem cells, expresses TRA-1-60 and TRA-1-81 antigens, and although their molecular identities are unknown, they are commonly used as markers of undifferentiated pluripotent human stem cells.
Product Information
Format
Purified
Control
Human embryonic stem cells
Presentation
Liquid in 0.05 M Potassium Phosphate, 0.3 M NaCl, pH 8.0 with 0.05% Sodium Azide.
Quality Level
MQ100
Applications
Application
Anti-TRA-1-81 Antibody, clone TRA-1-81 detects level of TRA-1-81 & has been published & validated for use in WB, FC, IF, IP & IC.
Key Applications
Western Blotting
Flow Cytometry
Immunofluorescence
Immunoprecipitation
Immunocytochemistry
Application Notes
Immunofluorescence: A previous lot of this antibody was used in IF.
Western Blot: A previous lot of this antibody was used in WB.
Immunoprecipitation: A previous lot of this antibody was used in IP.
Immunohistochemistry: A previous lot of this antibody was used in IH.
Flow Cytometry: A starting range of 10-20 µg/mL is suggested.
Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen
Human embryonal carcinoma cell line 2102Ep
Clone
clone TRA-1-81
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
This antibody reacts with TRA-1-81 antigen that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is seen with murine EC, EG or ES cells. Both the TRA-1-81 and TRA-1-60 monoclonal antibodies (MAB4360) recognize antigens that are associated with a pericellular matrix proteoglycan. TRA-1-60 reacts with a sialidase-sensitive epitope whilst TRA-1-81 reacts with an unknown epitope of the same molecule.
Isotype
IgM
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Gene Symbol
TRA-1-81
Stem Cell Type
Human Embryonic Stem Cells
Induced Pluripotent Stem Cells
Molecular Weight
420/250 kDa
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
