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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | IP, WB, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB4709 |
| Replaces | 04-443 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Caspase 9 Antibody, clone 2-23 |
| Alternate Names |
|
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified immunoglobulin by protein A. Liquid in PBS, pH 7.6, with 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Caspase 9 Antibody, clone 2-23 is an antibody against Caspase 9 for use in IP, WB, IH(P). |
| Key Applications |
|
| Application Notes | Western blot: 1:1000 Immunohistochemistry (frozen and paraffin): 1:500 - 1:1000 Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant human caspase-9 prodomain |
| Clone | 2-23 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Recognizes 46-48 kDa full-length procaspase-9, human recombinant caspase-9 and procaspase-9 from human cell lysates. Highly specific to caspase-9 and shows no cross-reaction with other caspase family members. Activation of procaspase-9 by Apaf-1 in the cytochrome c pathway requires proteolytic cleavage to generate active caspase-9. Once activated, capsase-9 initiates a caspase cascade involving the downstream executioner caspases 3, 6 and 7. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two transcript variants which encode different isoforms. |
| Gene Symbol |
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| Non-Reactive Species |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P55211 # Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9. SIZE: 416 amino acids; 46281 Da SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 35 kDa (p35) and a 10 kDa (p10) subunit. Caspase-9 and APAF1 bind to each other via their respective NH2-terminal CED-3 homologous domains in the presence of cytochrome C and ATP. Interacts with the inhibitors BIRC2, BIRC4, BIRC5 and BIRC7. TISSUE SPECIFICITY: Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. PTM: Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase- 3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events. SIMILARITY: SwissProt: P55211 ## Belongs to the peptidase C14 family. & Contains 1 CARD domain. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |