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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, Ch, M, R, Rb, Fg, Shk | IP, WB, ICC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB5200 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Synaptotagmin I Antibody |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified immunoglobulin. Liquid in PBS with 50% glycerol and 0.09% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Synaptotagmin I Antibody is validated for use in IP, WB, IC for the detection of Synaptotagmin I. |
| Key Applications |
|
| Application Notes | Western blot: 5 μg/mL (colormetric) Immunocytochemistry Immunoprecipitation Optimal working dilutions and protocols must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Rat brain synaptotagmin. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Synaptotagmin 1, also known as p65. By Western blot MAB5200 reacts with a single protein with a molecular weight of approximately 65 kDa. MAB5200 has not been epitope mapped. |
| Isotype | IgG2a |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM] |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P21579 # May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2. COFACTOR: Binds 3 calcium ions per subunit. The ions are bound to the C2 domains (By similarity). SIZE: 422 amino acids; 47573 Da SUBUNIT: Homotetramer (Probable). Interacts with STN2, SV2A, SV2B, SV2C and RIMS1. Forms a complex with SV2B, syntaxin 1 and SNAP25 (By similarity). SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle. Cytoplasmic vesicle, secretory vesicle, chromaffin granule. Note=Synaptic vesicles and chromaffin granules. DOMAIN: SwissProt: P21579 The first C2 domain mediates Ca(2+)-dependent phospholipid binding. & The second C2 domain mediates interaction with SV2A and STN2 (By similarity). SIMILARITY: Belongs to the synaptotagmin family. & Contains 2 C2 domains. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots up to 12 months.Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 200 µg |