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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | IP, WB, ICC, IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB5212 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Neurocan Antibody, clone 650.24 |
| Alternate Names |
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| Background Information | Neurocan is the major soluble chondroitin sulfate proteoglycan in the brain. It is thought to play a functional role in axonal growth and guidance and in the establishment of specific neural pathways during embryonic brain development. Neurocan expression in the brain is developmentally regulated. Early on the major form of neurocan consists of a 245 kD core protein with approximately two chondroitin sulfate glycosoaminoglycan chains of 22 kD each. Later neurocan comprises a 180 kD core protein. Both forms of neurocan contain only chondroitin 4-sulfate glycosoaminoglycan chains. By virtue of their high expression at sites of neurnal damage and trauma, chondroitin sulfate proteoglycans, including neurocan, are thought to inhibit successful nerve regeneration. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Neurocan Antibody, clone 650.24 is validated for use in IP, WB, IC, IH for the detection of Neurocan. |
| Key Applications |
|
| Application Notes | Western blot: 1-2 μg/mL. Reacts with polypeptides of 260 and 160 kDa on western blots of embryonic rat brain tissue extracts. The 160 kDa species is typically only seen aftyer chondroitinase treatment.Treatment is at a concentration of chondroitinase of 10U/mL in Tris-HCL pH 8.0. Make tissue or cell extract in 20-50mM Tris pH 7.6-8.0 with 0.15M NaCl in the presence of protease inhibitors. Add 1 microliter of enzyme to 30 microliters of extract and incubate 30 minutes at 37C. Then add SDS sample buffer, heat or boil sample as normal for SDS reducing samples. Immunocytochemistry: 1:5 Immunohistochemistry on 4% paraformaldehyde fixed tissue: 1:1,000 Immunoprecipitation: 1-2 μg/mL Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Embryonic rat brain proteoglycans |
| Clone | 650.24 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Neurocan. The specificity of clone 650.24 was checked by western blots of brain lysates and purified neurocan; the antibody reacted with the identical bands that clones 1D1 and 1F6 (other neurocan monoclonals) reacted; furthermore, the staining pattern of brain sections with these antibodies were all identical. Finally 650.24 also recognized recombinant neurocan expressed in transfected 293 cells by western blot. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Neurocan is a chondroitin sulfate proteoglycan thought to be involved in the modulation of cell adhesion and migration.[supplied by OMIM] |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: O14594 # May modulate neuronal adhesion and neurite growth during development by binding to neural cell adhesion molecules (NG-CAM and N-CAM). Chondroitin sulfate proteoglycan; binds to hyaluronic acid. SIZE: 1321 amino acids; 143093 Da TISSUE SPECIFICITY: Brain. SIMILARITY: SwissProt: O14594 ## Belongs to the aggrecan/versican proteoglycan family. & Contains 1 C-type lectin domain. & Contains 2 EGF-like domains. & Contains 1 Ig-like V-type (immunoglobulin-like) domain. & Contains 2 Link domains. & Contains 1 Sushi (CCP/SCR) domain. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at 2-8°C in undiluted aliquots for up to 6 months. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |