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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Av, B, F, H, M, R | FC, ICC, IHC, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB5258-20UG |
| Replaces | 04-1019 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Synaptophysin Antibody, clone SY38 |
| Alternate Names |
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| Background Information | Synaptophysin is a 38 kDa glycoprotein present in the membrane of neuronal presynaptic vesicles in brain, spinal cord, retina, vesicles of adrenal medulla, neuromuscular junctions, and endocrine cells. It is also expressed by neuroendocrine cells throughout the body, both normal and neoplastic. Synaptophysin is a useful marker for the identification of normal neuroendocrine cells and neuroendocrine neoplasms. This antibody reacts with neuroendocrine cells of human adrenal medulla, carotid body, skin, pituitary gland, thyroid, lung, pancreas, and gastointestinal mucosa. It also reacts with a wide spectrum of neuroendocrine neoplasms of neural type including neuroblastomas, ganglioneuroblastomas, ganglioneuromas, pheochromocytomas, chromaffin, and non chromaffin paragangliomas. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Purified mouse monoclonal IgG1κ in buffer containing 20 mM sodium phosphate buffer, 0.25 M NaCl, containing 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Synaptophysin using this Anti-Synaptophysin Antibody, clone SY38 validated for use in FC, IC, IH & WB. |
| Key Applications |
|
| Application Notes | Flow Cytometry (FACS): A previous lot of this antibody was used in Flow Cytometry. Western Blot: (Provoda, 2000) Reacts with a 38 kDa transmembrane glycoprotein Immunohistochemistry: For relatively cytoplasm-rich neuroendocrinic tumors a final concentration of 1 µg/mL is recommended. Concerning cytoplasm deficient tumors a concentration of 2 µg/mL should be used. Ideal frozen sections (4-5 mm) are obtained from shock-frozen tissue samples. The frozen sections are air-dried and then fixed with acetone for 5-10 min at -20°C. Excess acetone is allowed to evaporate at room temperature. Material fixed in alcohol or formalin and embedded in paraffin can also be used. It is advantageous to block unspecific binding sites by overlaying the sections with fetal calf serum for 20-30 min at room temperature. Excess of fetal calf serum is removed by decanting before application of the anti-body solution. Cytocentrifuge preparations of single cells or cell smears are also fixed in acetone. These preparations should but should be followed directly by antibody treatment. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Vesicular fraction of bovine brain. The SY38 epitope is to a pentapeptide repeat structure in the carboxy-terminal cytoplasmic tail of synaptophysin (Knaus & Betz 1990). |
| Clone | SY38 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | The antibody reacts with presynaptic vesicles of cerebral and spinal neurons, of neuromuscular endplates and with the retina of man, cow, rat and mouse. The antibody also reacts with vesicles of adrenal medulla and islet cells, and additionally allows specific staining of neuronal, adrenal and neuroepithelial tumors, such as pheochromocytoma, paraganglioma, islet cell tumors (incl. Insulinoma), medullary thyroid carcinoma and diverse pulmonary and gastro-intestinal carcinoids. The antibody also stains neurosecretory vesicles of certain culture cells, e.g. of the rat cell line PC-12. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Synaptophysin (p38) is an integral membrane protein of small synaptic vesicles in brain and endocrine cells.[supplied by OMIM] |
| Gene Symbol |
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| Purification Method | Protein A Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P08247 # Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. SIZE: 313 amino acids; 33845 Da SUBUNIT: Homohexamer or homotetramer. SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype. DOMAIN: SwissProt: P08247 The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein. PTM: Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation (By similarity). SIMILARITY: Belongs to the synaptophysin/synaptobrevin family. & Contains 1 MARVEL domain. |
| Molecular Weight | 38 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Western Blot on mouse brain lysates. Western Blot Analysis: 1:1000 dilution of this lot detected synaptophysin on 10 μg of mouse brain lysates. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 6 months at 2-8ºC from date of receipt. DO NOT FREEZE. |
| Packaging Information | |
|---|---|
| Material Size | 20 µg |